Biosynthesis of trypsinlike and chymotrypsinlike enzymes in immature Lutzomyia anthophora (Diptera: Psychodidae)

The biosynthesis of trypsinlike and chymotrypsinlike enzymes was followed in the four instars and pupa of Lutzomyia anthophora Addis. A 32-fold increase in the biosynthesis of trypsinlike enzymes was observed from the first to the fourth instar. Trypsinlike and chymotrypsinlike isozymes were also synthesized by pupae 1-8 d old. Similarly, a 29-fold increase in the biosynthesis of chymotrypsinlike isozymes also was observed from first to fourth instars. Several different [1,3-(3)H]DIP trypsinlike and chymotrypsinlike derivatives of first to fourth instars and pupae (1 and 8 d old) were studied using polyacrylamide gel electrophoresis and fluorography