Crystallization and preliminary X-ray study of minor glucoamylase from Aspergillus awamori variant X-100/D27
Crystals of the reduced form of glucoamylase were obtained from polyethylene glycol 6000 solution by the hanging-drop method. The protein was treated with α-mannosidase to partly remove the sugar component. The crystals belong to the space group P2 12 12 1 with cell dimensions a = 116.7 A ̊ , b = 10...
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| Veröffentlicht in: | Journal of molecular biology 1992-07, Vol.226 (1), p.271-272 |
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| container_title | Journal of molecular biology |
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| creator | Golubev, A.M. Neustroev, K.N. Aleshin, A.E. Firsov, L.M. |
| description | Crystals of the reduced form of glucoamylase were obtained from polyethylene glycol 6000 solution by the hanging-drop method. The protein was treated with α-mannosidase to partly remove the sugar component. The crystals belong to the space group
P2
12
12
1 with cell dimensions
a = 116.7
A
̊
, b = 104.3
A
̊
, c = 48.5
A
̊
and diffract beyond 2.5 Å resolution. |
| doi_str_mv | 10.1016/0022-2836(92)90139-B |
| format | Article |
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P2
12
12
1 with cell dimensions
a = 116.7
A
̊
, b = 104.3
A
̊
, c = 48.5
A
̊
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P2
12
12
1 with cell dimensions
a = 116.7
A
̊
, b = 104.3
A
̊
, c = 48.5
A
̊
and diffract beyond 2.5 Å resolution.</description><subject>alpha-Mannosidase</subject><subject>Aspergillus - enzymology</subject><subject>Aspergillus awamori</subject><subject>Biological and medical sciences</subject><subject>crystal structure</subject><subject>Crystalline structure</subject><subject>Crystallization</subject><subject>crystals</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glucan 1,4- alpha -glucosidase</subject><subject>Glucan 1,4-alpha-Glucosidase - chemistry</subject><subject>Glucan 1,4-alpha-Glucosidase - metabolism</subject><subject>glucoamylase</subject><subject>Mannosidases - metabolism</subject><subject>Molecular biophysics</subject><subject>molecular conformation</subject><subject>Polyethylene Glycols</subject><subject>Structure in molecular biology</subject><subject>X-ray</subject><subject>X-Ray Diffraction</subject><issn>0022-2836</issn><issn>1089-8638</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EKtvCPwDhA0JwCPVH4tgXpHb5lCpxgErcrIntrIyceGsnReHX12FX5QankWeeef3qHYSeUfKWEirOCWGsYpKL14q9UYRyVV0-QBtKpKqk4PIh2twjj9Fpzj8JIQ2v5Qk6oYIq0YgNCtu05AlC8L9h8nHEMFq8Ty74wY-QFvyjSrDgPM12wbHHpRsT3oXZRBiWANnhPsUBX-S9Szsfwpwx_IIhJo9vIXkYpyJBCTl_z9on6FEPIbunx3qGrj9--L79XF19_fRle3FVmbqmU0WhVn1nuTBcgIBOONkJq8BJ1fPylLC6Z7apG9W2wljFQXXSGs4BOqD8DL066O5TvJldnvTgs3EhwOjinHXLCVdM1P8FqahJS1lbwPoAmhRzTq7X--SHko-mRK_X0GvUeo1aK6b_XENflrXnR_25G5z9u3SIv8xfHueQDYQ-wWh8vscaLjiVrGAvDlgPUcMuFeT6Gyt_ENo2gtWrv3cHwpVYb71LOhvvRuOsT85M2kb_b6d3gXqvyQ</recordid><startdate>19920705</startdate><enddate>19920705</enddate><creator>Golubev, A.M.</creator><creator>Neustroev, K.N.</creator><creator>Aleshin, A.E.</creator><creator>Firsov, L.M.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19920705</creationdate><title>Crystallization and preliminary X-ray study of minor glucoamylase from Aspergillus awamori variant X-100/D27</title><author>Golubev, A.M. ; Neustroev, K.N. ; Aleshin, A.E. ; Firsov, L.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-1a49fbd36c36a6ab6e8b6d9ae89f3ab68a61962d5459776cd93a9b8dc33aaba13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>alpha-Mannosidase</topic><topic>Aspergillus - enzymology</topic><topic>Aspergillus awamori</topic><topic>Biological and medical sciences</topic><topic>crystal structure</topic><topic>Crystalline structure</topic><topic>Crystallization</topic><topic>crystals</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>glucan 1,4- alpha -glucosidase</topic><topic>Glucan 1,4-alpha-Glucosidase - chemistry</topic><topic>Glucan 1,4-alpha-Glucosidase - metabolism</topic><topic>glucoamylase</topic><topic>Mannosidases - metabolism</topic><topic>Molecular biophysics</topic><topic>molecular conformation</topic><topic>Polyethylene Glycols</topic><topic>Structure in molecular biology</topic><topic>X-ray</topic><topic>X-Ray Diffraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Golubev, A.M.</creatorcontrib><creatorcontrib>Neustroev, K.N.</creatorcontrib><creatorcontrib>Aleshin, A.E.</creatorcontrib><creatorcontrib>Firsov, L.M.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Golubev, A.M.</au><au>Neustroev, K.N.</au><au>Aleshin, A.E.</au><au>Firsov, L.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Crystallization and preliminary X-ray study of minor glucoamylase from Aspergillus awamori variant X-100/D27</atitle><jtitle>Journal of molecular biology</jtitle><addtitle>J Mol Biol</addtitle><date>1992-07-05</date><risdate>1992</risdate><volume>226</volume><issue>1</issue><spage>271</spage><epage>272</epage><pages>271-272</pages><issn>0022-2836</issn><eissn>1089-8638</eissn><coden>JMOBAK</coden><abstract>Crystals of the reduced form of glucoamylase were obtained from polyethylene glycol 6000 solution by the hanging-drop method. The protein was treated with α-mannosidase to partly remove the sugar component. The crystals belong to the space group
P2
12
12
1 with cell dimensions
a = 116.7
A
̊
, b = 104.3
A
̊
, c = 48.5
A
̊
and diffract beyond 2.5 Å resolution.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>1619656</pmid><doi>10.1016/0022-2836(92)90139-B</doi><tpages>2</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-2836 |
| ispartof | Journal of molecular biology, 1992-07, Vol.226 (1), p.271-272 |
| issn | 0022-2836 1089-8638 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73039264 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | alpha-Mannosidase Aspergillus - enzymology Aspergillus awamori Biological and medical sciences crystal structure Crystalline structure Crystallization crystals Fundamental and applied biological sciences. Psychology glucan 1,4- alpha -glucosidase Glucan 1,4-alpha-Glucosidase - chemistry Glucan 1,4-alpha-Glucosidase - metabolism glucoamylase Mannosidases - metabolism Molecular biophysics molecular conformation Polyethylene Glycols Structure in molecular biology X-ray X-Ray Diffraction |
| title | Crystallization and preliminary X-ray study of minor glucoamylase from Aspergillus awamori variant X-100/D27 |
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