Crystallization and preliminary X-ray study of minor glucoamylase from Aspergillus awamori variant X-100/D27

Crystals of the reduced form of glucoamylase were obtained from polyethylene glycol 6000 solution by the hanging-drop method. The protein was treated with α-mannosidase to partly remove the sugar component. The crystals belong to the space group P2 12 12 1 with cell dimensions a = 116.7 A ̊ , b = 10...

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Veröffentlicht in:	Journal of molecular biology 1992-07, Vol.226 (1), p.271-272
Hauptverfasser:	Golubev, A.M., Neustroev, K.N., Aleshin, A.E., Firsov, L.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha-Mannosidase Aspergillus - enzymology Aspergillus awamori Biological and medical sciences crystal structure Crystalline structure Crystallization crystals Fundamental and applied biological sciences. Psychology glucan 1,4- alpha -glucosidase Glucan 1,4-alpha-Glucosidase - chemistry Glucan 1,4-alpha-Glucosidase - metabolism glucoamylase Mannosidases - metabolism Molecular biophysics molecular conformation Polyethylene Glycols Structure in molecular biology X-ray X-Ray Diffraction
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Beschreibung
Zusammenfassung:	Crystals of the reduced form of glucoamylase were obtained from polyethylene glycol 6000 solution by the hanging-drop method. The protein was treated with α-mannosidase to partly remove the sugar component. The crystals belong to the space group P2 12 12 1 with cell dimensions a = 116.7 A ̊ , b = 104.3 A ̊ , c = 48.5 A ̊ and diffract beyond 2.5 Å resolution.
ISSN:	0022-2836 1089-8638
DOI:	10.1016/0022-2836(92)90139-B