Dinitrophenyl (DNP) hapten sandwich staining (DHSS) procedure: A 10 year review of its principle reagents and applications

Over the past 10 years an immunoperoxidase method using dinitrophenyl (DNP) hapten-labelled primary or secondary probes has been devised. Its widely successful application in research and diagnostic work has depended upon the development of certain key reagents. These include a novel non-deleterious...

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Veröffentlicht in:	Journal of immunological methods 1992-06, Vol.150 (1), p.193-198
Hauptverfasser:	Jasani, B., Thomas, N.D., Navabi, H., Millar, D.M., Newman, G.R., Gee, J., Williams, E.D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Monoclonal Biological and medical sciences Biotin Densitometry Dinitrobenzenes Dinitrophenyl Dinitrophenyl localization system Dinitrophenyl-hapten procedure Fundamental and applied biological sciences. Psychology Fundamental immunology Haptens Immunoassay Immunoenzyme Techniques Immunoglobulin M Immunolocalization Immunoperoxidase Indicators and Reagents Molecular immunology Peroxidase Techniques
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Beschreibung
Zusammenfassung:	Over the past 10 years an immunoperoxidase method using dinitrophenyl (DNP) hapten-labelled primary or secondary probes has been devised. Its widely successful application in research and diagnostic work has depended upon the development of certain key reagents. These include a novel non-deleterious DNP labelling compound, a unique multivalent monoclonal bridge antibody, and an efficient DNP hapten substituted or anti-DNP linked marker enzyme. In this article the development of these reagents and various modifications of the basic technique are reviewed in conjunction with the special applications accruing from their use.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/0022-1759(92)90078-8