A cosmid vector containing a dominant selectable marker for cloning Chlamydomonas genes by complementation

A cosmid vector containing a dominant selectable marker for cloning Chlamydomonas genes by complementation

Cosmid vectors containing a dominate selectable marker ( ble) for complementation cloning of genes in Chlamydomonas reinhardtii were created. The usefulness of these vectors, which differ in the orientation of the ble cassette, was demonstrated by transforming C. reinhardtii to phleomycin resistance...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Plasmid 2003, Vol.49 (1), p.75-78
Hauptverfasser: Chang, Mary, Li, Fei, Odom, O.W., Lee, Jaesung, Herrin, David L.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Bacterial Proteins - genetics
ble cassette
Chlamydomonas
Chlamydomonas reinhardtii - drug effects
Chlamydomonas reinhardtii - genetics
Cloning, Molecular - methods
Complementation cloning
Cosmid vector
Cosmids - genetics
Dominant marker
Gene Library
Genes, Dominant - genetics
Genetic Complementation Test
Genetic Markers
Genetic Vectors
Phleomycin resistance
Phleomycins - pharmacology
Selection, Genetic
Transformation, Genetic
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Cosmid vectors containing a dominate selectable marker ( ble) for complementation cloning of genes in Chlamydomonas reinhardtii were created. The usefulness of these vectors, which differ in the orientation of the ble cassette, was demonstrated by transforming C. reinhardtii to phleomycin resistance, by constructing a large library (∼5×10 5 recombinants) in one of them using DNA from a C. reinhardtii mutant, and by transforming C. reinhardtii with recombinant cosmid clones and pools.
ISSN:0147-619X
1095-9890
DOI:10.1016/S0147-619X(02)00158-0