Oxidative cleavage of DNA by tridentate copper (II) complex

Copper (II) complex 1 having planar tridentate ligand, bzimpy, where bzimpy is 2,6-bis(benzimidazo-2-yl) pyridine was synthesized and characterized by UV–visible, FAB (fast atom bombardment) mass and infrared spectroscopy. From absorption titration data, the binding constant of Cu(II) with DNA was c...

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Veröffentlicht in:	Journal of inorganic biochemistry 2003-01, Vol.93 (3), p.271-276
Hauptverfasser:	Vaidyanathan, Vaidyanathan Ganesan, Nair, Balachandran Unni
Format:	Artikel
Sprache:	eng
Schlagworte:	Copper (II) complex Copper - chemistry DNA - chemistry DNA - metabolism Intercalating Agents - chemistry Intercalating Agents - pharmacology Ligands Mass Spectrometry Nucleic Acid Denaturation - drug effects Organometallic Compounds - chemistry Organometallic Compounds - pharmacology Oxidation-Reduction Oxidative cleavage Plasmids - chemistry Plasmids - metabolism Spectrum Analysis Synthesis Temperature Tridentate planar complex
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Beschreibung
Zusammenfassung:	Copper (II) complex 1 having planar tridentate ligand, bzimpy, where bzimpy is 2,6-bis(benzimidazo-2-yl) pyridine was synthesized and characterized by UV–visible, FAB (fast atom bombardment) mass and infrared spectroscopy. From absorption titration data, the binding constant of Cu(II) with DNA was calculated to be (1.8±0.02)×10 4 M −1. Thermal denaturation study of DNA with 1 revealed Δ T m of 5±0.5 °C. Viscosity measurement showed that complex binds with DNA through intercalative mode. Copper (II) complex induces cleavage in plasmid DNA in the presence of coreductants such as ascorbic acid or glutathione.
ISSN:	0162-0134 1873-3344
DOI:	10.1016/S0162-0134(02)00593-7