Extraction and characterisation of the intact form of bovine vitreous type IX collagen

We provide the first biochemical characterisation of intact type IX collagen extracted from bovine vitreous. It possesses a shortened α1(IX) chain (Mr 64K) compared to its cartilage counterpart (Mr 84K). All the vitreous type IX collagen is in a proteoglycan form, its glycosaminoglycan constituent b...

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Veröffentlicht in:	Biochemical and biophysical research communications 1992-05, Vol.185 (1), p.392-397
Hauptverfasser:	Bishop, Paul, McLeod, David, Ayad, Shirley
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Blotting, Western Cattle Centrifugation, Isopycnic Chondroitin Sulfates - chemistry Collagen - chemistry Collagen - immunology Collagen - isolation & purification Dermatan Sulfate - chemistry Fundamental and applied biological sciences. Psychology Glycoproteins Proteins Proteoglycans - chemistry Vitreous Body - chemistry Vitreous Body - immunology
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Zusammenfassung:	We provide the first biochemical characterisation of intact type IX collagen extracted from bovine vitreous. It possesses a shortened α1(IX) chain (Mr 64K) compared to its cartilage counterpart (Mr 84K). All the vitreous type IX collagen is in a proteoglycan form, its glycosaminoglycan constituent being a chondroitin/dermatan sulphate component of Mr 15–60K attached to the α2(IX) chain. This contrasts with previous findings in chick vitreous where a very long glycosaminoglycan chain of Mr∼350K was demonstrated.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/S0006-291X(05)80998-2