A simple method for the extraction of baculovirus DNA

A simple method for the extraction of baculovirus DNA

We have developed a method using lysozyme for DNA extraction from Baculoviruses using as a model Lymantria dispar nuclear polyhedrosis virus (LdNPV) obtained from infected larvae. This method proved to be quick, inexpensive and the extracted DNA was successfully used in molecular hybridization exper...

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Veröffentlicht in:Journal of virological methods 1992-05, Vol.37 (2), p.235-240
Hauptverfasser: Desmarteaux, D., Charpentier, G., Arella, M.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Baculoviridae - genetics
Baculovirus
Biological and medical sciences
DNA extraction
DNA, Viral - isolation & purification
Endopeptidase K
Fundamental and applied biological sciences. Psychology
Larva - microbiology
Lysozyme
Microbiology
Moths - microbiology
Muramidase
nuclear polyhedrosis virus
Serine Endopeptidases
Techniques used in virology
Virology
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Beschreibung
Zusammenfassung:We have developed a method using lysozyme for DNA extraction from Baculoviruses using as a model Lymantria dispar nuclear polyhedrosis virus (LdNPV) obtained from infected larvae. This method proved to be quick, inexpensive and the extracted DNA was successfully used in molecular hybridization experiments.
ISSN:0166-0934
1879-0984
DOI:10.1016/0166-0934(92)90050-N