Modeling the performance of pilot-scale countercurrent chromatography: Scale-up predictions and experimental verification of erythromycin separation

Biosynthesis of polyketide antibiotics, such as erythromycin A (EA), can result in the formation of analogues of the main compound that are chemically and structurally extremely similar. The large‐scale purification of these antibiotics by conventional high‐performance liquid chromatography (HPLC) c...

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Veröffentlicht in:	Biotechnology and bioengineering 2003-03, Vol.81 (6), p.640-649
Hauptverfasser:	Booth, A.J., Sutherland, I. A., Lye, G. J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Chromatography, Liquid - instrumentation Chromatography, Liquid - methods Computer Simulation Equipment Design erythromycin Erythromycin - analogs & derivatives Erythromycin - chemistry Erythromycin - isolation & purification Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Models, Chemical Others Pilot Projects pilot-scale countercurrent chromatography predictive scale-up Quality Control Sensitivity and Specificity Various methods and equipments
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Zusammenfassung:	Biosynthesis of polyketide antibiotics, such as erythromycin A (EA), can result in the formation of analogues of the main compound that are chemically and structurally extremely similar. The large‐scale purification of these antibiotics by conventional high‐performance liquid chromatography (HPLC) can be prohibitively expensive due to the large volume of both solvent and adsorbent required. This study examines the feasibility of using a novel pilot‐scale countercurrent chromatography (CCC) machine as an alternative to HPLC. CCC is a low‐pressure (typically
ISSN:	0006-3592 1097-0290
DOI:	10.1002/bit.10508