Role of protein kinase C (PKC) in bone resorption: Effect of the specific PKC inhibitor 1-alkyl-2-methylglycerol
The specific inhibitor of protein kinase C, 1-O-alkyl-2-O-methylglycerol (AMG), was studied for its effect on bone resorption, measured as 45Ca-release, in fetal mouse calvariae. AMG (1 to 50 μM) had no effect on basal bone resorption. AMG inhibited parathyroid hormone (40 nM) induced bone resorptio...
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Veröffentlicht in: | Biochemical and biophysical research communications 1992-05, Vol.184 (3), p.1317-1323 |
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Sprache: | eng |
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Zusammenfassung: | The specific inhibitor of protein kinase C, 1-O-alkyl-2-O-methylglycerol (AMG), was studied for its effect on bone resorption, measured as
45Ca-release, in fetal mouse calvariae. AMG (1 to 50
μM) had no effect on basal bone resorption. AMG inhibited parathyroid hormone (40 nM) induced bone resorption in a dose-dependent manner. Resorption induced by 1,25 (OH)
2-vitamin D
3 (10 nM) or prostaglandin E
2 (5
μM) was also inhibited by AMG. The release of β-glucuronidase activity paralled the course of the
45Ca-release. The production of interleukin 6, induced by parathyroid hormone, in fetal rat calvarial osteoblasts was not affected by AMG. AMG (1 to 50
μM) had no cytotoxic effects on cells or calvariae. From these results it is concluded that protein kinase C may have an important role in the regulation of bone resorption. |
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ISSN: | 0006-291X 1090-2104 |
DOI: | 10.1016/S0006-291X(05)80026-9 |