Saccharomyces cerevisiae cells secreting an Aspergillus niger beta-galactosidase grow on whey permeate
We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase ( lacA ) from Aspergillus niger . Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total...
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| Veröffentlicht in: | Bio/Technology 1992-01, Vol.10 (1), p.82-85 |
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| creator | Kumar, V. (London Hospital Medical School, London, U.K.) Ramakrishnan, S Teeri, T.T Knowles, J.K.C Hartley, B.S |
| description | We describe the construction of a lactose-utilizing
Saccharomyces cerevisiae
that expresses the cDNA for a secreted, thermostable β-galactosidase (
lacA
) from
Aspergillus niger
. Yeast cells expressing the
lacA
gene from the yeast
ADH1
promoter on a multicopy plasmid secrete up to 40% of the total P-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the
lacA
gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal. |
| doi_str_mv | 10.1038/nbt0192-82 |
| format | Article |
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Saccharomyces cerevisiae
that expresses the cDNA for a secreted, thermostable β-galactosidase (
lacA
) from
Aspergillus niger
. Yeast cells expressing the
lacA
gene from the yeast
ADH1
promoter on a multicopy plasmid secrete up to 40% of the total P-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the
lacA
gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.</description><identifier>ISSN: 0733-222X</identifier><identifier>ISSN: 1087-0156</identifier><identifier>EISSN: 2331-3684</identifier><identifier>EISSN: 1546-1696</identifier><identifier>DOI: 10.1038/nbt0192-82</identifier><identifier>PMID: 1368193</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; Agriculture ; Alcohol Dehydrogenase - genetics ; Amino Acid Sequence ; ASPERGILLUS NIGER ; Aspergillus niger - enzymology ; Aspergillus niger - genetics ; Base Sequence ; BETA GALACTOSIDASA ; BETA GALACTOSIDASE ; beta-Galactosidase - genetics ; beta-Galactosidase - metabolism ; Bioinformatics ; Biological and medical sciences ; BIOLOGICAL TREATMENT ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; Biomedicine ; Biotechnology ; CARBOHYDRATE METABOLISM ; CLONACION ; CLONAGE ; CLONING ; Cloning, Molecular - methods ; Culture Media ; DNA, Fungal - genetics ; DNA, Fungal - isolation & purification ; ENZYMIC ACTIVITY ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fundamental and applied biological sciences. Psychology ; GENE ; GENE EXPRESSION ; GENE TRANSFER ; GENES ; Genes, Fungal ; Genetic engineering ; Genetic technics ; GENETIC TRANSFORMATION ; Genetic Vectors ; Kinetics ; LACA GENE ; LACTOSA ; LACTOSE ; LACTOSERUM ; Life Sciences ; METABOLISME DES GLUCIDES ; METABOLISMO DE CARBOHIDRATOS ; Methods. Procedures. Technologies ; Milk Proteins ; MOLECULAR SEQUENCE DATA ; NUCLEOTIDE ; NUCLEOTIDES ; NUCLEOTIDOS ; PLASMIDE ; PLASMIDIOS ; PLASMIDS ; Promoter Regions, Genetic ; PROTEINAS ; PROTEINE ; PROTEINS ; Recombinant Proteins - metabolism ; research-paper ; SACCHAROMYCES CEREVISIAE ; Saccharomyces cerevisiae - enzymology ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - growth & development ; SECRECION ; SECRETION ; SUERO DE LA LECHE ; TRANSFERENCIA DE GENES ; TRANSFERT DE GENE ; TRANSFORMACION GENETICA ; TRANSFORMATION GENETIQUE ; Vectors (cloning, transfer, expression). Insertion sequences and transposons ; WHEY ; Whey Proteins</subject><ispartof>Bio/Technology, 1992-01, Vol.10 (1), p.82-85</ispartof><rights>Nature Publishing Company 1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c323t-58084a08d4408b724a5b69a3499c20cc721f8ad2e06420b6aa1e8188f61995533</citedby><cites>FETCH-LOGICAL-c323t-58084a08d4408b724a5b69a3499c20cc721f8ad2e06420b6aa1e8188f61995533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/nbt0192-82$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/nbt0192-82$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,2727,4024,27923,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5524798$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1368193$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kumar, V. (London Hospital Medical School, London, U.K.)</creatorcontrib><creatorcontrib>Ramakrishnan, S</creatorcontrib><creatorcontrib>Teeri, T.T</creatorcontrib><creatorcontrib>Knowles, J.K.C</creatorcontrib><creatorcontrib>Hartley, B.S</creatorcontrib><title>Saccharomyces cerevisiae cells secreting an Aspergillus niger beta-galactosidase grow on whey permeate</title><title>Bio/Technology</title><addtitle>Nat Biotechnol</addtitle><addtitle>Biotechnology (N Y)</addtitle><description>We describe the construction of a lactose-utilizing
Saccharomyces cerevisiae
that expresses the cDNA for a secreted, thermostable β-galactosidase (
lacA
) from
Aspergillus niger
. Yeast cells expressing the
lacA
gene from the yeast
ADH1
promoter on a multicopy plasmid secrete up to 40% of the total P-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the
lacA
gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.</description><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>Agriculture</subject><subject>Alcohol Dehydrogenase - genetics</subject><subject>Amino Acid Sequence</subject><subject>ASPERGILLUS NIGER</subject><subject>Aspergillus niger - enzymology</subject><subject>Aspergillus niger - genetics</subject><subject>Base Sequence</subject><subject>BETA GALACTOSIDASA</subject><subject>BETA GALACTOSIDASE</subject><subject>beta-Galactosidase - genetics</subject><subject>beta-Galactosidase - metabolism</subject><subject>Bioinformatics</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL TREATMENT</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>CARBOHYDRATE METABOLISM</subject><subject>CLONACION</subject><subject>CLONAGE</subject><subject>CLONING</subject><subject>Cloning, Molecular - methods</subject><subject>Culture Media</subject><subject>DNA, Fungal - genetics</subject><subject>DNA, Fungal - isolation & purification</subject><subject>ENZYMIC ACTIVITY</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>GENE EXPRESSION</subject><subject>GENE TRANSFER</subject><subject>GENES</subject><subject>Genes, Fungal</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>GENETIC TRANSFORMATION</subject><subject>Genetic Vectors</subject><subject>Kinetics</subject><subject>LACA GENE</subject><subject>LACTOSA</subject><subject>LACTOSE</subject><subject>LACTOSERUM</subject><subject>Life Sciences</subject><subject>METABOLISME DES GLUCIDES</subject><subject>METABOLISMO DE CARBOHIDRATOS</subject><subject>Methods. Procedures. Technologies</subject><subject>Milk Proteins</subject><subject>MOLECULAR SEQUENCE DATA</subject><subject>NUCLEOTIDE</subject><subject>NUCLEOTIDES</subject><subject>NUCLEOTIDOS</subject><subject>PLASMIDE</subject><subject>PLASMIDIOS</subject><subject>PLASMIDS</subject><subject>Promoter Regions, Genetic</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>PROTEINS</subject><subject>Recombinant Proteins - metabolism</subject><subject>research-paper</subject><subject>SACCHAROMYCES CEREVISIAE</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - growth & development</subject><subject>SECRECION</subject><subject>SECRETION</subject><subject>SUERO DE LA LECHE</subject><subject>TRANSFERENCIA DE GENES</subject><subject>TRANSFERT DE GENE</subject><subject>TRANSFORMACION GENETICA</subject><subject>TRANSFORMATION GENETIQUE</subject><subject>Vectors (cloning, transfer, expression). Insertion sequences and transposons</subject><subject>WHEY</subject><subject>Whey Proteins</subject><issn>0733-222X</issn><issn>1087-0156</issn><issn>2331-3684</issn><issn>1546-1696</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkc1r3DAQxUVpSbdpLz0WCjqUHlqc6su2dAyhXxDIIQn0ZsbaseNgS1uN3bD_fbR4SS49jeD99B7zhrH3UpxJoe230M5COlVY9YJtlNay0JU1L9lG1FoXSqk_r9kbonshTF0pc8JOZAak0xvWXYP3d5DitPdI3GPCfwMNgPk5jsQJfcJ5CD2HwM9ph6kfxnEhHoYeE29xhqKHEfwcadgCIe9TfOAx8Ic73PPMTwgzvmWvOhgJ3x3nKbv98f3m4ldxefXz98X5ZeG10nNRWmENCLs1Rti2VgbKtnKgjXNeCe9rJTsLW4WiMkq0FYBEK63tKulcWWp9yj6vvrsU_y5IczMNdNgEAsaFmlq5WuYPGfyygj5FooRds0vDBGnfSNEcSm2OpTZWZfjj0XVpJ9w-o2uLWf901IE8jF2C4Ad6wspSmdodMr-uGGUl5Pqa-7ikkPv4fyhf6QDzkvDJ7fnYGfmwIh3EBvqUM2-vnayEUFI_AolpoOQ</recordid><startdate>199201</startdate><enddate>199201</enddate><creator>Kumar, V. (London Hospital Medical School, London, U.K.)</creator><creator>Ramakrishnan, S</creator><creator>Teeri, T.T</creator><creator>Knowles, J.K.C</creator><creator>Hartley, B.S</creator><general>Nature Publishing Group US</general><general>Nature Publications</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199201</creationdate><title>Saccharomyces cerevisiae cells secreting an Aspergillus niger beta-galactosidase grow on whey permeate</title><author>Kumar, V. (London Hospital Medical School, London, U.K.) ; Ramakrishnan, S ; Teeri, T.T ; Knowles, J.K.C ; Hartley, B.S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c323t-58084a08d4408b724a5b69a3499c20cc721f8ad2e06420b6aa1e8188f61995533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>ACTIVIDAD ENZIMATICA</topic><topic>ACTIVITE ENZYMATIQUE</topic><topic>Agriculture</topic><topic>Alcohol Dehydrogenase - genetics</topic><topic>Amino Acid Sequence</topic><topic>ASPERGILLUS NIGER</topic><topic>Aspergillus niger - enzymology</topic><topic>Aspergillus niger - genetics</topic><topic>Base Sequence</topic><topic>BETA GALACTOSIDASA</topic><topic>BETA GALACTOSIDASE</topic><topic>beta-Galactosidase - genetics</topic><topic>beta-Galactosidase - metabolism</topic><topic>Bioinformatics</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL TREATMENT</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering/Biotechnology</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>CARBOHYDRATE METABOLISM</topic><topic>CLONACION</topic><topic>CLONAGE</topic><topic>CLONING</topic><topic>Cloning, Molecular - methods</topic><topic>Culture Media</topic><topic>DNA, Fungal - genetics</topic><topic>DNA, Fungal - isolation & purification</topic><topic>ENZYMIC ACTIVITY</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>GENE EXPRESSION</topic><topic>GENE TRANSFER</topic><topic>GENES</topic><topic>Genes, Fungal</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>GENETIC TRANSFORMATION</topic><topic>Genetic Vectors</topic><topic>Kinetics</topic><topic>LACA GENE</topic><topic>LACTOSA</topic><topic>LACTOSE</topic><topic>LACTOSERUM</topic><topic>Life Sciences</topic><topic>METABOLISME DES GLUCIDES</topic><topic>METABOLISMO DE CARBOHIDRATOS</topic><topic>Methods. Procedures. Technologies</topic><topic>Milk Proteins</topic><topic>MOLECULAR SEQUENCE DATA</topic><topic>NUCLEOTIDE</topic><topic>NUCLEOTIDES</topic><topic>NUCLEOTIDOS</topic><topic>PLASMIDE</topic><topic>PLASMIDIOS</topic><topic>PLASMIDS</topic><topic>Promoter Regions, Genetic</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>PROTEINS</topic><topic>Recombinant Proteins - metabolism</topic><topic>research-paper</topic><topic>SACCHAROMYCES CEREVISIAE</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - growth & development</topic><topic>SECRECION</topic><topic>SECRETION</topic><topic>SUERO DE LA LECHE</topic><topic>TRANSFERENCIA DE GENES</topic><topic>TRANSFERT DE GENE</topic><topic>TRANSFORMACION GENETICA</topic><topic>TRANSFORMATION GENETIQUE</topic><topic>Vectors (cloning, transfer, expression). Insertion sequences and transposons</topic><topic>WHEY</topic><topic>Whey Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kumar, V. (London Hospital Medical School, London, U.K.)</creatorcontrib><creatorcontrib>Ramakrishnan, S</creatorcontrib><creatorcontrib>Teeri, T.T</creatorcontrib><creatorcontrib>Knowles, J.K.C</creatorcontrib><creatorcontrib>Hartley, B.S</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bio/Technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kumar, V. (London Hospital Medical School, London, U.K.)</au><au>Ramakrishnan, S</au><au>Teeri, T.T</au><au>Knowles, J.K.C</au><au>Hartley, B.S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Saccharomyces cerevisiae cells secreting an Aspergillus niger beta-galactosidase grow on whey permeate</atitle><jtitle>Bio/Technology</jtitle><stitle>Nat Biotechnol</stitle><addtitle>Biotechnology (N Y)</addtitle><date>1992-01</date><risdate>1992</risdate><volume>10</volume><issue>1</issue><spage>82</spage><epage>85</epage><pages>82-85</pages><issn>0733-222X</issn><issn>1087-0156</issn><eissn>2331-3684</eissn><eissn>1546-1696</eissn><abstract>We describe the construction of a lactose-utilizing
Saccharomyces cerevisiae
that expresses the cDNA for a secreted, thermostable β-galactosidase (
lacA
) from
Aspergillus niger
. Yeast cells expressing the
lacA
gene from the yeast
ADH1
promoter on a multicopy plasmid secrete up to 40% of the total P-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the
lacA
gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>1368193</pmid><doi>10.1038/nbt0192-82</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0733-222X |
| ispartof | Bio/Technology, 1992-01, Vol.10 (1), p.82-85 |
| issn | 0733-222X 1087-0156 2331-3684 1546-1696 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72971188 |
| source | MEDLINE; Nature; SpringerLink Journals - AutoHoldings |
| subjects | ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Agriculture Alcohol Dehydrogenase - genetics Amino Acid Sequence ASPERGILLUS NIGER Aspergillus niger - enzymology Aspergillus niger - genetics Base Sequence BETA GALACTOSIDASA BETA GALACTOSIDASE beta-Galactosidase - genetics beta-Galactosidase - metabolism Bioinformatics Biological and medical sciences BIOLOGICAL TREATMENT Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology CARBOHYDRATE METABOLISM CLONACION CLONAGE CLONING Cloning, Molecular - methods Culture Media DNA, Fungal - genetics DNA, Fungal - isolation & purification ENZYMIC ACTIVITY EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology GENE GENE EXPRESSION GENE TRANSFER GENES Genes, Fungal Genetic engineering Genetic technics GENETIC TRANSFORMATION Genetic Vectors Kinetics LACA GENE LACTOSA LACTOSE LACTOSERUM Life Sciences METABOLISME DES GLUCIDES METABOLISMO DE CARBOHIDRATOS Methods. Procedures. Technologies Milk Proteins MOLECULAR SEQUENCE DATA NUCLEOTIDE NUCLEOTIDES NUCLEOTIDOS PLASMIDE PLASMIDIOS PLASMIDS Promoter Regions, Genetic PROTEINAS PROTEINE PROTEINS Recombinant Proteins - metabolism research-paper SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development SECRECION SECRETION SUERO DE LA LECHE TRANSFERENCIA DE GENES TRANSFERT DE GENE TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE Vectors (cloning, transfer, expression). Insertion sequences and transposons WHEY Whey Proteins |
| title | Saccharomyces cerevisiae cells secreting an Aspergillus niger beta-galactosidase grow on whey permeate |
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