Saccharomyces cerevisiae cells secreting an Aspergillus niger beta-galactosidase grow on whey permeate

We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase ( lacA ) from Aspergillus niger . Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total...

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Veröffentlicht in:	Bio/Technology 1992-01, Vol.10 (1), p.82-85
Hauptverfasser:	Kumar, V. (London Hospital Medical School, London, U.K.), Ramakrishnan, S, Teeri, T.T, Knowles, J.K.C, Hartley, B.S
Format:	Artikel
Sprache:	eng
Schlagworte:	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Agriculture Alcohol Dehydrogenase - genetics Amino Acid Sequence ASPERGILLUS NIGER Aspergillus niger - enzymology Aspergillus niger - genetics Base Sequence BETA GALACTOSIDASA BETA GALACTOSIDASE beta-Galactosidase - genetics beta-Galactosidase - metabolism Bioinformatics Biological and medical sciences BIOLOGICAL TREATMENT Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology CARBOHYDRATE METABOLISM CLONACION CLONAGE CLONING Cloning, Molecular - methods Culture Media DNA, Fungal - genetics DNA, Fungal - isolation & purification ENZYMIC ACTIVITY EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology GENE GENE EXPRESSION GENE TRANSFER GENES Genes, Fungal Genetic engineering Genetic technics GENETIC TRANSFORMATION Genetic Vectors Kinetics LACA GENE LACTOSA LACTOSE LACTOSERUM Life Sciences METABOLISME DES GLUCIDES METABOLISMO DE CARBOHIDRATOS Methods. Procedures. Technologies Milk Proteins MOLECULAR SEQUENCE DATA NUCLEOTIDE NUCLEOTIDES NUCLEOTIDOS PLASMIDE PLASMIDIOS PLASMIDS Promoter Regions, Genetic PROTEINAS PROTEINE PROTEINS Recombinant Proteins - metabolism research-paper SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development SECRECION SECRETION SUERO DE LA LECHE TRANSFERENCIA DE GENES TRANSFERT DE GENE TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE Vectors (cloning, transfer, expression). Insertion sequences and transposons WHEY Whey Proteins
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Beschreibung
Zusammenfassung:	We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase ( lacA ) from Aspergillus niger . Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total P-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.
ISSN:	0733-222X 1087-0156 2331-3684 1546-1696
DOI:	10.1038/nbt0192-82