Effects of endurance training on the gluconeogenic capacity of periportal and perivenous hepatocytes
This study was undertaken to assess the effect of 8 weeks of endurance training (treadmill, last 4 weeks: 60 min at 26 m/min, 8–10% grade) on the gluconeogenic capacity of periportal (PP-H) and perivenous (PV-H) hepatocytes of overnight fasted rats. Isolated PP-H and PV-H, obtained by selective dest...
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| Veröffentlicht in: | Physiology & behavior 2003, Vol.78 (1), p.27-32 |
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| creator | Bélanger, Patrice Désy, François Gauthier, Marie-Soleil Couturier, Karine Lavoie, Jean-Marc |
| description | This study was undertaken to assess the effect of 8 weeks of endurance training (treadmill, last 4 weeks: 60 min at 26 m/min, 8–10% grade) on the gluconeogenic capacity of periportal (PP-H) and perivenous (PV-H) hepatocytes of overnight fasted rats. Isolated PP-H and PV-H, obtained by selective destruction after liver perfusion with digitonin and collagenase, were incubated with saturating concentrations of a mixture of lactate and pyruvate (20:2 mM; Lac+Pyr) or alanine (20 mM; Ala) to determine the glucose production flux (
J
glucose) in the incubation medium. Endogenous
J
glucose as well as
J
glucose from substrates were significantly higher (
P |
| doi_str_mv | 10.1016/S0031-9384(02)00887-9 |
| format | Article |
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J
glucose) in the incubation medium. Endogenous
J
glucose as well as
J
glucose from substrates were significantly higher (
P<.05) in PP-H than PV-H in the untrained state. Following training, a selective increase (
P<.05) in
J
glucose from endogenous substrates and from Lac+Pyr was observed in PV-H only, resulting in the disappearance (
P>.05) of the difference of
J
glucose between PP-H and PV-H. It is concluded that the increase in the gluconeogenic capacity of the liver following endurance training is first observed in PV-H.</description><identifier>ISSN: 0031-9384</identifier><identifier>EISSN: 1873-507X</identifier><identifier>DOI: 10.1016/S0031-9384(02)00887-9</identifier><identifier>PMID: 12536007</identifier><language>eng</language><publisher>Cambridge: Elsevier Inc</publisher><subject>Alanine - metabolism ; Animals ; Biological and medical sciences ; Carbohydrates ; Cell Separation ; Female ; Fundamental and applied biological sciences. Psychology ; Gluconeogenesis ; Gluconeogenesis - physiology ; Glucose flux ; Glutamate-Ammonia Ligase - metabolism ; Hepatic Veins - physiology ; Hepatic zonation ; Hepatocytes - metabolism ; Kinetics ; Lactic Acid - metabolism ; Liver ; Metabolisms and neurohumoral controls ; Physical Conditioning, Animal - physiology ; Physical Endurance - physiology ; Portal Vein - physiology ; Pyruvic Acid - metabolism ; Rats ; Rats, Sprague-Dawley ; Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><ispartof>Physiology & behavior, 2003, Vol.78 (1), p.27-32</ispartof><rights>2003 Elsevier Science Inc.</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0031-9384(02)00887-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14514593$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12536007$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bélanger, Patrice</creatorcontrib><creatorcontrib>Désy, François</creatorcontrib><creatorcontrib>Gauthier, Marie-Soleil</creatorcontrib><creatorcontrib>Couturier, Karine</creatorcontrib><creatorcontrib>Lavoie, Jean-Marc</creatorcontrib><title>Effects of endurance training on the gluconeogenic capacity of periportal and perivenous hepatocytes</title><title>Physiology & behavior</title><addtitle>Physiol Behav</addtitle><description>This study was undertaken to assess the effect of 8 weeks of endurance training (treadmill, last 4 weeks: 60 min at 26 m/min, 8–10% grade) on the gluconeogenic capacity of periportal (PP-H) and perivenous (PV-H) hepatocytes of overnight fasted rats. Isolated PP-H and PV-H, obtained by selective destruction after liver perfusion with digitonin and collagenase, were incubated with saturating concentrations of a mixture of lactate and pyruvate (20:2 mM; Lac+Pyr) or alanine (20 mM; Ala) to determine the glucose production flux (
J
glucose) in the incubation medium. Endogenous
J
glucose as well as
J
glucose from substrates were significantly higher (
P<.05) in PP-H than PV-H in the untrained state. Following training, a selective increase (
P<.05) in
J
glucose from endogenous substrates and from Lac+Pyr was observed in PV-H only, resulting in the disappearance (
P>.05) of the difference of
J
glucose between PP-H and PV-H. It is concluded that the increase in the gluconeogenic capacity of the liver following endurance training is first observed in PV-H.</description><subject>Alanine - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carbohydrates</subject><subject>Cell Separation</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gluconeogenesis</subject><subject>Gluconeogenesis - physiology</subject><subject>Glucose flux</subject><subject>Glutamate-Ammonia Ligase - metabolism</subject><subject>Hepatic Veins - physiology</subject><subject>Hepatic zonation</subject><subject>Hepatocytes - metabolism</subject><subject>Kinetics</subject><subject>Lactic Acid - metabolism</subject><subject>Liver</subject><subject>Metabolisms and neurohumoral controls</subject><subject>Physical Conditioning, Animal - physiology</subject><subject>Physical Endurance - physiology</subject><subject>Portal Vein - physiology</subject><subject>Pyruvic Acid - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>0031-9384</issn><issn>1873-507X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoMo7jj6E5S-KHporXx0On0SWXZXYcGDCt5CJl2ZjfQkbZJemH9v5gP3uEWgKHjeSvG-hLym8JEClZ9-AHDaDlyJ98A-ACjVt8MTsqKq520H_e-nZPUfuSAvcv4Dtbjgz8kFZR2XAP2KjFfOoS25ia7BMC7JBItNScYHH7ZNDE25w2Y7LTYGjFsM3jbWzMb6sj9oZkx-jqmYqTFhPI73GOKSmzucTYl2XzC_JM-cmTK-Ovc1-XV99fPya3v7_ebb5Zfb1vIeSisUZ9LhaKURkg8jFUqpDW64McCEE1R1GymkZLavreucYYoyyoSlDqQAvibvTnvnFP8umIve-Wxxmky9fcm6Z0MV0sdBqiTvBmAV7E6gTTHnhE7Pye9M2msK-pCDPuagDyZrYPqYQ53W5M35g2Wzw_FBdTa-Am_PgMnWTO7gu88PnOjqG3jlPp84rL7de0w6W481o9Gnmpseo3_klH8zm6Sm</recordid><startdate>2003</startdate><enddate>2003</enddate><creator>Bélanger, Patrice</creator><creator>Désy, François</creator><creator>Gauthier, Marie-Soleil</creator><creator>Couturier, Karine</creator><creator>Lavoie, Jean-Marc</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TS</scope><scope>7X8</scope></search><sort><creationdate>2003</creationdate><title>Effects of endurance training on the gluconeogenic capacity of periportal and perivenous hepatocytes</title><author>Bélanger, Patrice ; Désy, François ; Gauthier, Marie-Soleil ; Couturier, Karine ; Lavoie, Jean-Marc</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-48326fedc6a4639d14888beb3aa024f4185b64662c764655fa2812124c1f06403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Alanine - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Carbohydrates</topic><topic>Cell Separation</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gluconeogenesis</topic><topic>Gluconeogenesis - physiology</topic><topic>Glucose flux</topic><topic>Glutamate-Ammonia Ligase - metabolism</topic><topic>Hepatic Veins - physiology</topic><topic>Hepatic zonation</topic><topic>Hepatocytes - metabolism</topic><topic>Kinetics</topic><topic>Lactic Acid - metabolism</topic><topic>Liver</topic><topic>Metabolisms and neurohumoral controls</topic><topic>Physical Conditioning, Animal - physiology</topic><topic>Physical Endurance - physiology</topic><topic>Portal Vein - physiology</topic><topic>Pyruvic Acid - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bélanger, Patrice</creatorcontrib><creatorcontrib>Désy, François</creatorcontrib><creatorcontrib>Gauthier, Marie-Soleil</creatorcontrib><creatorcontrib>Couturier, Karine</creatorcontrib><creatorcontrib>Lavoie, Jean-Marc</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Physical Education Index</collection><collection>MEDLINE - Academic</collection><jtitle>Physiology & behavior</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bélanger, Patrice</au><au>Désy, François</au><au>Gauthier, Marie-Soleil</au><au>Couturier, Karine</au><au>Lavoie, Jean-Marc</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of endurance training on the gluconeogenic capacity of periportal and perivenous hepatocytes</atitle><jtitle>Physiology & behavior</jtitle><addtitle>Physiol Behav</addtitle><date>2003</date><risdate>2003</risdate><volume>78</volume><issue>1</issue><spage>27</spage><epage>32</epage><pages>27-32</pages><issn>0031-9384</issn><eissn>1873-507X</eissn><abstract>This study was undertaken to assess the effect of 8 weeks of endurance training (treadmill, last 4 weeks: 60 min at 26 m/min, 8–10% grade) on the gluconeogenic capacity of periportal (PP-H) and perivenous (PV-H) hepatocytes of overnight fasted rats. Isolated PP-H and PV-H, obtained by selective destruction after liver perfusion with digitonin and collagenase, were incubated with saturating concentrations of a mixture of lactate and pyruvate (20:2 mM; Lac+Pyr) or alanine (20 mM; Ala) to determine the glucose production flux (
J
glucose) in the incubation medium. Endogenous
J
glucose as well as
J
glucose from substrates were significantly higher (
P<.05) in PP-H than PV-H in the untrained state. Following training, a selective increase (
P<.05) in
J
glucose from endogenous substrates and from Lac+Pyr was observed in PV-H only, resulting in the disappearance (
P>.05) of the difference of
J
glucose between PP-H and PV-H. It is concluded that the increase in the gluconeogenic capacity of the liver following endurance training is first observed in PV-H.</abstract><cop>Cambridge</cop><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>12536007</pmid><doi>10.1016/S0031-9384(02)00887-9</doi><tpages>6</tpages></addata></record> |
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| subjects | Alanine - metabolism Animals Biological and medical sciences Carbohydrates Cell Separation Female Fundamental and applied biological sciences. Psychology Gluconeogenesis Gluconeogenesis - physiology Glucose flux Glutamate-Ammonia Ligase - metabolism Hepatic Veins - physiology Hepatic zonation Hepatocytes - metabolism Kinetics Lactic Acid - metabolism Liver Metabolisms and neurohumoral controls Physical Conditioning, Animal - physiology Physical Endurance - physiology Portal Vein - physiology Pyruvic Acid - metabolism Rats Rats, Sprague-Dawley Vertebrates: anatomy and physiology, studies on body, several organs or systems |
| title | Effects of endurance training on the gluconeogenic capacity of periportal and perivenous hepatocytes |
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