Effects of endurance training on the gluconeogenic capacity of periportal and perivenous hepatocytes

This study was undertaken to assess the effect of 8 weeks of endurance training (treadmill, last 4 weeks: 60 min at 26 m/min, 8–10% grade) on the gluconeogenic capacity of periportal (PP-H) and perivenous (PV-H) hepatocytes of overnight fasted rats. Isolated PP-H and PV-H, obtained by selective dest...

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Veröffentlicht in:	Physiology & behavior 2003, Vol.78 (1), p.27-32
Hauptverfasser:	Bélanger, Patrice, Désy, François, Gauthier, Marie-Soleil, Couturier, Karine, Lavoie, Jean-Marc
Format:	Artikel
Sprache:	eng
Schlagworte:	Alanine - metabolism Animals Biological and medical sciences Carbohydrates Cell Separation Female Fundamental and applied biological sciences. Psychology Gluconeogenesis Gluconeogenesis - physiology Glucose flux Glutamate-Ammonia Ligase - metabolism Hepatic Veins - physiology Hepatic zonation Hepatocytes - metabolism Kinetics Lactic Acid - metabolism Liver Metabolisms and neurohumoral controls Physical Conditioning, Animal - physiology Physical Endurance - physiology Portal Vein - physiology Pyruvic Acid - metabolism Rats Rats, Sprague-Dawley Vertebrates: anatomy and physiology, studies on body, several organs or systems
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Zusammenfassung:	This study was undertaken to assess the effect of 8 weeks of endurance training (treadmill, last 4 weeks: 60 min at 26 m/min, 8–10% grade) on the gluconeogenic capacity of periportal (PP-H) and perivenous (PV-H) hepatocytes of overnight fasted rats. Isolated PP-H and PV-H, obtained by selective destruction after liver perfusion with digitonin and collagenase, were incubated with saturating concentrations of a mixture of lactate and pyruvate (20:2 mM; Lac+Pyr) or alanine (20 mM; Ala) to determine the glucose production flux ( J glucose) in the incubation medium. Endogenous J glucose as well as J glucose from substrates were significantly higher ( P
ISSN:	0031-9384 1873-507X
DOI:	10.1016/S0031-9384(02)00887-9