Chemiluminescence Detection for a Microchip Capillary Electrophoresis System Fabricated in Poly(dimethylsiloxane)
Chemiluminescence (CL) detection integrated with a microchip capillary electrophoresis (MCE) system that was fabricated in poly(dimethylsiloxane) was demonstrated for chemical and biochemical analyses. Two model CL systems were involved here: metal ion-catalyzed luminol−peroxide reaction and dansyl...
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Veröffentlicht in: | Analytical chemistry (Washington) 2003-01, Vol.75 (1), p.36-41 |
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description | Chemiluminescence (CL) detection integrated with a microchip capillary electrophoresis (MCE) system that was fabricated in poly(dimethylsiloxane) was demonstrated for chemical and biochemical analyses. Two model CL systems were involved here: metal ion-catalyzed luminol−peroxide reaction and dansyl species conjugated peroxalate−peroxide reaction. Different strategies based on three chip patterns (cross, cross combining with Y, and cross combining with V) to perform on-line CL detection for MCE were evaluated and compared in terms of sensitivity, reproducibility, and peak symmetry. The chip pattern of cross combining with Y proved to be promising for the luminol−peroxide CL system, while the chip pattern of cross combining with V was preferred for the peroxalate−peroxide system where CL reagent could not be effectively transported by electroosmotic flow. A detection limit down to submicromolar concentrations (midattomole) was achieved with good reproducibility and symmetric peak shape. Successful separation of three metal cations such as Cr(III), Co(II), and Cu(II) and chiral recognition of dansyl phenylalanine enantiomers within 1 min revealed distinct advantages of combining MCE with CL detection for rapid and sensitive analyses. |
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Two model CL systems were involved here: metal ion-catalyzed luminol−peroxide reaction and dansyl species conjugated peroxalate−peroxide reaction. Different strategies based on three chip patterns (cross, cross combining with Y, and cross combining with V) to perform on-line CL detection for MCE were evaluated and compared in terms of sensitivity, reproducibility, and peak symmetry. The chip pattern of cross combining with Y proved to be promising for the luminol−peroxide CL system, while the chip pattern of cross combining with V was preferred for the peroxalate−peroxide system where CL reagent could not be effectively transported by electroosmotic flow. A detection limit down to submicromolar concentrations (midattomole) was achieved with good reproducibility and symmetric peak shape. Successful separation of three metal cations such as Cr(III), Co(II), and Cu(II) and chiral recognition of dansyl phenylalanine enantiomers within 1 min revealed distinct advantages of combining MCE with CL detection for rapid and sensitive analyses.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac026096s</identifier><identifier>PMID: 12530816</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analytical chemistry ; Biochemistry ; Chemical and thermal methods ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Exact sciences and technology ; Other chromatographic methods ; Scientific imaging ; Semiconductors</subject><ispartof>Analytical chemistry (Washington), 2003-01, Vol.75 (1), p.36-41</ispartof><rights>Copyright © 2003 American Chemical Society</rights><rights>2003 INIST-CNRS</rights><rights>Copyright American Chemical Society Jan 1, 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a472t-98ab44e57c51e720a26bc9a9d60a51c5b3adc56584e22005653b2f5ca307a25a3</citedby><cites>FETCH-LOGICAL-a472t-98ab44e57c51e720a26bc9a9d60a51c5b3adc56584e22005653b2f5ca307a25a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac026096s$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac026096s$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14474056$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12530816$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Bi-Feng</creatorcontrib><creatorcontrib>Ozaki, Motoaki</creatorcontrib><creatorcontrib>Utsumi, Yuichi</creatorcontrib><creatorcontrib>Hattori, Tadashi</creatorcontrib><creatorcontrib>Terabe, Shigeru</creatorcontrib><title>Chemiluminescence Detection for a Microchip Capillary Electrophoresis System Fabricated in Poly(dimethylsiloxane)</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Chemiluminescence (CL) detection integrated with a microchip capillary electrophoresis (MCE) system that was fabricated in poly(dimethylsiloxane) was demonstrated for chemical and biochemical analyses. Two model CL systems were involved here: metal ion-catalyzed luminol−peroxide reaction and dansyl species conjugated peroxalate−peroxide reaction. Different strategies based on three chip patterns (cross, cross combining with Y, and cross combining with V) to perform on-line CL detection for MCE were evaluated and compared in terms of sensitivity, reproducibility, and peak symmetry. The chip pattern of cross combining with Y proved to be promising for the luminol−peroxide CL system, while the chip pattern of cross combining with V was preferred for the peroxalate−peroxide system where CL reagent could not be effectively transported by electroosmotic flow. A detection limit down to submicromolar concentrations (midattomole) was achieved with good reproducibility and symmetric peak shape. Successful separation of three metal cations such as Cr(III), Co(II), and Cu(II) and chiral recognition of dansyl phenylalanine enantiomers within 1 min revealed distinct advantages of combining MCE with CL detection for rapid and sensitive analyses.</description><subject>Analytical chemistry</subject><subject>Biochemistry</subject><subject>Chemical and thermal methods</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Exact sciences and technology</subject><subject>Other chromatographic methods</subject><subject>Scientific imaging</subject><subject>Semiconductors</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNpl0d9rFDEQB_Agij2rD_4DEgTFPqxOspsf-1iurQqnnrTSxzCbzXGpu5ttsgu9_97IHT3QpwzkwzDzHUJeM_jIgLNPaIFLqGV6QhZMcCik1vwpWQBAWXAFcEJepHQHwBgw-ZycMC5K0EwuyP1y63rfzb0fXLJusI5euMnZyYeBbkKkSL95G4Pd-pEucfRdh3FHL7tMYhi3IbrkE73epcn19Aqb6C1OrqV-oOvQ7T60vnfTdtcl34UHHNzZS_Jsg11yrw7vKfl1dXmz_FKsfnz-ujxfFVgpPhW1xqaqnFBWMKc4IJeNrbFuJaBgVjQltlZIoSvHOUCuyoZvhMUSFHKB5Sl5v-87xnA_uzSZ3ucN8_yDC3MyitcStNYZvv0H3oU5Dnk2w5nStWSSZ3S2RzmLlKLbmDH6PkdhGJi_RzCPR8j2zaHh3PSuPcpD6hm8OwBMFrtNxMH6dHRVpaq8UXbF3vmc7sPjP8bfRqpSCXOzvjY_b1f6dn3x3ayOfdGm4xL_D_gHe9mqog</recordid><startdate>20030101</startdate><enddate>20030101</enddate><creator>Liu, Bi-Feng</creator><creator>Ozaki, Motoaki</creator><creator>Utsumi, Yuichi</creator><creator>Hattori, Tadashi</creator><creator>Terabe, Shigeru</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20030101</creationdate><title>Chemiluminescence Detection for a Microchip Capillary Electrophoresis System Fabricated in Poly(dimethylsiloxane)</title><author>Liu, Bi-Feng ; 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Chem</addtitle><date>2003-01-01</date><risdate>2003</risdate><volume>75</volume><issue>1</issue><spage>36</spage><epage>41</epage><pages>36-41</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>Chemiluminescence (CL) detection integrated with a microchip capillary electrophoresis (MCE) system that was fabricated in poly(dimethylsiloxane) was demonstrated for chemical and biochemical analyses. Two model CL systems were involved here: metal ion-catalyzed luminol−peroxide reaction and dansyl species conjugated peroxalate−peroxide reaction. Different strategies based on three chip patterns (cross, cross combining with Y, and cross combining with V) to perform on-line CL detection for MCE were evaluated and compared in terms of sensitivity, reproducibility, and peak symmetry. 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subjects | Analytical chemistry Biochemistry Chemical and thermal methods Chemistry Chromatographic methods and physical methods associated with chromatography Exact sciences and technology Other chromatographic methods Scientific imaging Semiconductors |
title | Chemiluminescence Detection for a Microchip Capillary Electrophoresis System Fabricated in Poly(dimethylsiloxane) |
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