Chemiluminescence Detection for a Microchip Capillary Electrophoresis System Fabricated in Poly(dimethylsiloxane)

Chemiluminescence (CL) detection integrated with a microchip capillary electrophoresis (MCE) system that was fabricated in poly(dimethylsiloxane) was demonstrated for chemical and biochemical analyses. Two model CL systems were involved here:  metal ion-catalyzed luminol−peroxide reaction and dansyl...

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Veröffentlicht in:Analytical chemistry (Washington) 2003-01, Vol.75 (1), p.36-41
Hauptverfasser: Liu, Bi-Feng, Ozaki, Motoaki, Utsumi, Yuichi, Hattori, Tadashi, Terabe, Shigeru
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creator Liu, Bi-Feng
Ozaki, Motoaki
Utsumi, Yuichi
Hattori, Tadashi
Terabe, Shigeru
description Chemiluminescence (CL) detection integrated with a microchip capillary electrophoresis (MCE) system that was fabricated in poly(dimethylsiloxane) was demonstrated for chemical and biochemical analyses. Two model CL systems were involved here:  metal ion-catalyzed luminol−peroxide reaction and dansyl species conjugated peroxalate−peroxide reaction. Different strategies based on three chip patterns (cross, cross combining with Y, and cross combining with V) to perform on-line CL detection for MCE were evaluated and compared in terms of sensitivity, reproducibility, and peak symmetry. The chip pattern of cross combining with Y proved to be promising for the luminol−peroxide CL system, while the chip pattern of cross combining with V was preferred for the peroxalate−peroxide system where CL reagent could not be effectively transported by electroosmotic flow. A detection limit down to submicromolar concentrations (midattomole) was achieved with good reproducibility and symmetric peak shape. Successful separation of three metal cations such as Cr(III), Co(II), and Cu(II) and chiral recognition of dansyl phenylalanine enantiomers within 1 min revealed distinct advantages of combining MCE with CL detection for rapid and sensitive analyses.
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subjects Analytical chemistry
Biochemistry
Chemical and thermal methods
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
Other chromatographic methods
Scientific imaging
Semiconductors
title Chemiluminescence Detection for a Microchip Capillary Electrophoresis System Fabricated in Poly(dimethylsiloxane)
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