Identification of an antigen-specific B cell population
The difficulty in characterizing antigen-specific B cells that arise in the native B cell repertoire has been a formidable obstacle to understanding both protective and pathogenic antibody responses. We have developed a tetramer-based technique for identifying antigen-specific B cells. Biotin-labele...
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| Veröffentlicht in: | Journal of immunological methods 2003-01, Vol.272 (1), p.177-187 |
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| container_end_page | 187 |
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| container_title | Journal of immunological methods |
| container_volume | 272 |
| creator | Newman, Jeffrey Rice, Jeffrey S. Wang, Chuansheng Harris, Shannon L. Diamond, Betty |
| description | The difficulty in characterizing antigen-specific B cells that arise in the native B cell repertoire has been a formidable obstacle to understanding both protective and pathogenic antibody responses. We have developed a tetramer-based technique for identifying antigen-specific B cells. Biotin-labeled antigen is made tetrameric by interaction with streptavidin. The enhanced avidity of this antigenic compound for the B cell membrane permits the visualization, characterization and isolation of antigen-specific B cells. |
| doi_str_mv | 10.1016/S0022-1759(02)00499-4 |
| format | Article |
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Psychology</topic><topic>Gene Expression</topic><topic>Humoral immune response</topic><topic>Immunization</topic><topic>In Vitro Techniques</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Motility, taxis</topic><topic>Polymerase Chain Reaction</topic><topic>Protein Structure, Quaternary</topic><topic>Spleen - cytology</topic><topic>Spleen - immunology</topic><topic>Streptavidin</topic><topic>Tetrameric antigen</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Newman, Jeffrey</creatorcontrib><creatorcontrib>Rice, Jeffrey S.</creatorcontrib><creatorcontrib>Wang, Chuansheng</creatorcontrib><creatorcontrib>Harris, Shannon L.</creatorcontrib><creatorcontrib>Diamond, Betty</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Newman, Jeffrey</au><au>Rice, Jeffrey S.</au><au>Wang, Chuansheng</au><au>Harris, Shannon L.</au><au>Diamond, Betty</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of an antigen-specific B cell population</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>2003-01-15</date><risdate>2003</risdate><volume>272</volume><issue>1</issue><spage>177</spage><epage>187</epage><pages>177-187</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>The difficulty in characterizing antigen-specific B cells that arise in the native B cell repertoire has been a formidable obstacle to understanding both protective and pathogenic antibody responses. 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| ispartof | Journal of immunological methods, 2003-01, Vol.272 (1), p.177-187 |
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| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Antigen-specific B cells Antigens - chemistry Antigens, CD - chemistry Antigens, CD - genetics Antigens, CD - metabolism B-Lymphocyte Subsets - immunology Bacteriology Biological and medical sciences Biotin CD79 Antigens Cell Membrane - immunology Female Flow Cytometry Fundamental and applied biological sciences. Psychology Gene Expression Humoral immune response Immunization In Vitro Techniques Mice Mice, Inbred BALB C Microbiology Motility, taxis Polymerase Chain Reaction Protein Structure, Quaternary Spleen - cytology Spleen - immunology Streptavidin Tetrameric antigen |
| title | Identification of an antigen-specific B cell population |
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