Identification of an antigen-specific B cell population

The difficulty in characterizing antigen-specific B cells that arise in the native B cell repertoire has been a formidable obstacle to understanding both protective and pathogenic antibody responses. We have developed a tetramer-based technique for identifying antigen-specific B cells. Biotin-labele...

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Veröffentlicht in:	Journal of immunological methods 2003-01, Vol.272 (1), p.177-187
Hauptverfasser:	Newman, Jeffrey, Rice, Jeffrey S., Wang, Chuansheng, Harris, Shannon L., Diamond, Betty
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antigen-specific B cells Antigens - chemistry Antigens, CD - chemistry Antigens, CD - genetics Antigens, CD - metabolism B-Lymphocyte Subsets - immunology Bacteriology Biological and medical sciences Biotin CD79 Antigens Cell Membrane - immunology Female Flow Cytometry Fundamental and applied biological sciences. Psychology Gene Expression Humoral immune response Immunization In Vitro Techniques Mice Mice, Inbred BALB C Microbiology Motility, taxis Polymerase Chain Reaction Protein Structure, Quaternary Spleen - cytology Spleen - immunology Streptavidin Tetrameric antigen
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Zusammenfassung:	The difficulty in characterizing antigen-specific B cells that arise in the native B cell repertoire has been a formidable obstacle to understanding both protective and pathogenic antibody responses. We have developed a tetramer-based technique for identifying antigen-specific B cells. Biotin-labeled antigen is made tetrameric by interaction with streptavidin. The enhanced avidity of this antigenic compound for the B cell membrane permits the visualization, characterization and isolation of antigen-specific B cells.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/S0022-1759(02)00499-4