Development of rainbow trout microsatellite markers from repeat enriched libraries

Development of rainbow trout microsatellite markers from repeat enriched libraries

The efficiency of developing polymorphic microsatellite markers from 2 repeat enriched libraries was evaluated. Thirty-six polymorphic microsatellite markers were developed for rainbow trout, 27 of which were informative in a mapping family. The ability of each marker to amplify genomic DNA from oth...

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Veröffentlicht in:Marine biotechnology (New York, N.Y.) N.Y.), 2002-01, Vol.4 (1), p.12-16
Hauptverfasser: Rexroad, 3rd, C E, Coleman, R L, Gustafson, A L, Hershberger, W K, Killefer, J
Format: Artikel
Sprache:eng
Schlagworte:
Brackish
Deoxyribonucleic acid
DNA
Fish
Freshwater
Freshwater fishes
Gene loci
Gene mapping
Genetic markers
Libraries
Mapping
Marine
Markers
Microsatellites
Oncorhynchus mykiss
Salmonids
Trout
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Zusammenfassung:The efficiency of developing polymorphic microsatellite markers from 2 repeat enriched libraries was evaluated. Thirty-six polymorphic microsatellite markers were developed for rainbow trout, 27 of which were informative in a mapping family. The ability of each marker to amplify genomic DNA from other salmonids was also observed.
ISSN:1436-2228
1436-2236
DOI:10.1007/s10126-001-0058-6