Thermostable and alkaline-tolerant cellulase-free xylanase produced by thermotolerant Streptomyces sp. Ab106

Cellulase-free xylanase was produced by Streptomyces sp. Ab106 using cane bagasse as the substrate at 55°C. Its maximum activity was 13 IU without cellulase and mannanase activities. Its profiles were investigated. Its optimum temperature and pH were 60°C and 6.0, respectively. More than 70% of its...

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Veröffentlicht in:Journal of bioscience and bioengineering 2002-04, Vol.93 (4), p.431-433
Hauptverfasser: Techapun, Charin, Charoenrat, Thanakorn, Poosaran, Naiyatat, Watanabe, Masanori, Sasak, Ken
Format: Artikel
Sprache:eng
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Zusammenfassung:Cellulase-free xylanase was produced by Streptomyces sp. Ab106 using cane bagasse as the substrate at 55°C. Its maximum activity was 13 IU without cellulase and mannanase activities. Its profiles were investigated. Its optimum temperature and pH were 60°C and 6.0, respectively. More than 70% of its activity was remained at 60°C at pH 9. This enzyme was quite stable and exhibited an active of more than 70% for 144 h at 60°C, and of more than 80% for 144 h at 40°C, pH 9. This thermo-tolerant and alkaline-tolerant xylanase can be used in the pulp bleaching process.
ISSN:1389-1723
1347-4421
DOI:10.1016/S1389-1723(02)80080-9