Partial amino acid sequence of a somatostatin receptor isolated from GH4C1 pituitary cells

A somatostatin receptor isolated from GH4C1 rat pituitary tumor-derived cells was cleaved with cyanogen bromide or cyanogen bromide+trypsin to obtain sequenceable fragments. Five unique amino acid sequences ranging from 6 to 27 amino acid residues were obtained. The sequence was identical to sequence recently reported for one of two somatostatin receptors cloned from human pancreas [Yamada et al., (1992) Proc. Natl. Acad. Sci. U.S.A.89, 251–255] except for a single valine to isoleucine substitution. This is the first report of amino acid sequence from a purified somatostatin receptor.