Testosterone destroys the transcortin-receptor complex

Dissociation of the complex of transcortin receptor with immobilized transcortin in the presence of 10−5 M testosterone has been shown with the use of affinity chromatography on transcortin-Sepharose. The specificity of this effect is confirmed by its abrogation in the presence of cortisol. The test...

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Veröffentlicht in:	Biochemical and biophysical research communications 1992-04, Vol.184 (1), p.491-497
Hauptverfasser:	Krupenko, Sergey A., Strel'chyonok, Oleg A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Cell Membrane - metabolism Cell physiology Chromatography, Affinity - methods complexes Decidua dissociation Electrophoresis, Polyacrylamide Gel Endometrium - metabolism Female Fundamental and applied biological sciences. Psychology Hormonal regulation Humans induction Isoelectric Focusing Molecular and cellular biology Molecular Weight Pregnancy receptors Receptors, Cell Surface - drug effects Receptors, Cell Surface - isolation & purification Receptors, Cell Surface - metabolism Serpins testosterone Testosterone - pharmacology transcortin Transcortin - metabolism
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Beschreibung
Zusammenfassung:	Dissociation of the complex of transcortin receptor with immobilized transcortin in the presence of 10−5 M testosterone has been shown with the use of affinity chromatography on transcortin-Sepharose. The specificity of this effect is confirmed by its abrogation in the presence of cortisol. The testosterone effect has been used for the elution of transcortin receptor from affinity column. The receptor retained transcortin-binding capacity after the elution and removal of testosterone. Characteristics of the receptor obtained by testosterone elution were identical with those of the transcortin eluted preparation.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/0006-291X(92)91221-B