Near-Field Optical Analysis of Living Cells in Vitro

Near-Field Optical Analysis of Living Cells in Vitro

Near-field optical analysis (NOA) provides morphological nanoscale mappings of living cells in liquid cell culture media and nondestructive insight into cell functionality. Here we show for the first time the performance of NOA in imaging living cells. Unlabeled human endothelial cells attached to p...

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Veröffentlicht in:Journal of proteome research 2002-03, Vol.1 (2), p.111-114
Hauptverfasser: Sommer, Andrei P, Franke, Ralf-Peter
Format: Artikel
Sprache:eng
Schlagworte:
Endothelium - ultrastructure
Humans
In Vitro Techniques
Microscopy
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Beschreibung
Zusammenfassung:Near-field optical analysis (NOA) provides morphological nanoscale mappings of living cells in liquid cell culture media and nondestructive insight into cell functionality. Here we show for the first time the performance of NOA in imaging living cells. Unlabeled human endothelial cells attached to polished titanium disks were analyzed with hydrophobically coated optical biosensors mounted to a near-field scanning optical microscope (NSOM). Biosensors and titanium substrates could be simply implemented in standard NSOM and high-throughput NOA. Keywords: NOA • NSOM • AFM • biosensors • titanium • cellular imaging
ISSN:1535-3893
1535-3907
DOI:10.1021/pr025501t