Structural and Functional Properties of Yersinia pestis Caf1 Capsular Antigen and Their Possible Role in Fulminant Development of Primary Pneumonic Plague

Yersinia pestis capsular antigen Caf1 is shown to be a β-structural protein that in polymeric form possesses very high conformational stability. Different approaches show that a dimer is the minimal cooperative block of Caf1 adhesin. Caf1 dimer interacts effectively with IL-1 receptors of human macr...

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Veröffentlicht in:	J.Proteome Res.1:307,2002 2002, 2002-07, Vol.1 (4), p.307-315
Hauptverfasser:	Abramov, Vyacheslav M, Vasiliev, Anatoly M, Khlebnikov, Valentin S, Vasilenko, Raisa N, Kulikova, Nataly L, Kosarev, Igor V, Ishchenko, Alexander T, Gillespie, Joel R, Millett, Ian S, Fink, Anthony L, Uversky, Vladimir N
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals ANTIGENS Antigens, Bacterial - chemistry Antigens, Bacterial - immunology Antigens, Bacterial - metabolism Bacterial Capsules - chemistry Bacterial Capsules - immunology Bacterial Capsules - metabolism Bacterial Proteins - chemistry Bacterial Proteins - metabolism Cell Line Circular Dichroism Dimerization Epithelial Cells - cytology Epithelial Cells - metabolism FUNCTIONALS Guanidine - chemistry Humans Hydrogen-Ion Concentration Macrophages - cytology Macrophages - metabolism Models, Biological PARTICLE ACCELERATORS Plague - immunology Plague - metabolism Plague - microbiology Protein Binding Protein Conformation Protein Denaturation Receptors, Interleukin-1 - metabolism Spectroscopy, Fourier Transform Infrared STANFORD LINEAR ACCELERATOR CENTER STANFORD SYNCHROTRON RADIATION LABORATORY SYNCHROTRON RADIATION Yersinia pestis - chemistry Yersinia pestis - immunology
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Beschreibung
Zusammenfassung:	Yersinia pestis capsular antigen Caf1 is shown to be a β-structural protein that in polymeric form possesses very high conformational stability. Different approaches show that a dimer is the minimal cooperative block of Caf1 adhesin. Caf1 dimer interacts effectively with IL-1 receptors of human macrophage and epithelial cells. The specificity of such interaction is confirmed by the inhibition of IL-1α binding by Caf1. The Caf1 role in pneumonic plague pathogenesis is discussed. Keywords: bubonic plague • pneumonic plague • capsular antigen Caf1 • molecular adhesin • conformational stability
ISSN:	1535-3893 1535-3907
DOI:	10.1021/pr025511u