Fatty acids rendered immunogenic

A method allowing the covalent binding of fatty acids to a carrier was developed. The absence of adsorbed fatty acids was controlled by HPTLC. Rabbits were immunized with these immunogens. The antibodies obtained were studied by ELISA and were shown to be directed against the fatty acyl chain, whate...

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Veröffentlicht in:	Immunology letters 1992-02, Vol.31 (3), p.227-232
Hauptverfasser:	Maneta-Peyret, Lilly, Picard, Jean-Paul, Bezian, Jean-Henri, Cassagne, Claude
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies Antibody Formation Antibody Specificity Antigenic determinants, haptens, artificial antigens Antigens Biological and medical sciences Chromatography, Thin Layer Drug Carriers ELISA Enzyme-Linked Immunosorbent Assay Fatty acids Fatty Acids - chemistry Fatty Acids - immunology Fundamental and applied biological sciences. Psychology Fundamental immunology HPTLC Ligands Molecular immunology Rabbits Serum Albumin, Bovine - chemistry
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Beschreibung
Zusammenfassung:	A method allowing the covalent binding of fatty acids to a carrier was developed. The absence of adsorbed fatty acids was controlled by HPTLC. Rabbits were immunized with these immunogens. The antibodies obtained were studied by ELISA and were shown to be directed against the fatty acyl chain, whatever its length.
ISSN:	0165-2478 1879-0542
DOI:	10.1016/0165-2478(92)90118-8