Effect of sperm-immobilizing antibodies on the acrosome reaction of human spermatozoa

To study by a triple stain technique the effect of sperm-immobilizing antibodies on the acrosome reaction of human spermatozoa. The spermatozoa were allowed to swim up and culture in a medium containing 7.5% (vol/vol) serum with sperm-immobilizing antibodies or control serum up to 6hours. Sperm mobi...

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Veröffentlicht in:	Fertility and sterility 1992-02, Vol.57 (2), p.387-392
Hauptverfasser:	Bandoh, Ritsuo, Yamano, Shuji, Kamada, Masaharu, Daitoh, Toshifumi, Aono, Toshihiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Acrosome - physiology acrosome reaction Antibodies - physiology Antisperm antibody Biological and medical sciences Birth control Cell Survival Gynecology. Andrology. Obstetrics human spermatozoa Humans infertility Male Medical sciences Sperm Motility - physiology sperm-immobilizing antibody Spermatozoa - physiology Staining and Labeling Sterility. Assisted procreation
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Beschreibung
Zusammenfassung:	To study by a triple stain technique the effect of sperm-immobilizing antibodies on the acrosome reaction of human spermatozoa. The spermatozoa were allowed to swim up and culture in a medium containing 7.5% (vol/vol) serum with sperm-immobilizing antibodies or control serum up to 6hours. Sperm mobility was analyzed, and the percentage of live acrosome reacted spermatozoa was determined. Samples were collected from patients referred to university hospital infertility clinics. Serum samples were drawn from seven patients with sperm-immobilizing antibodies. All the sera were heat inactivated and stored at −40°C until use. Semen samples were taken from two healthy donors. During culture for 6hours, the percentage of live sperm showing the acrosome reaction increased significantly (P
ISSN:	0015-0282 1556-5653
DOI:	10.1016/S0015-0282(16)54851-1