Synthesis and characterization of a d(ApG) platinated nonanucleotide duplex

The nonamer 5′d(CTCAGCCTC) 3′ 1 has been reacted with cis-diamminediaquaplatinum(II) in water at pH 4.2. The major reaction product was shown by enzymatic digestion and 1H NMR to be the d(ApG) cis-Pt(NH 3) 2 chelate [ cis-Pt(NH 3) 2{d(CTCAGCCTC)- N7(4), N7(5)}] 1-Pt. When mixed with its complementar...

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Veröffentlicht in:	Biochemical and biophysical research communications 1992-01, Vol.182 (2), p.555-560
Hauptverfasser:	Fouchet, M.H., Gauthier, C., Guittet, E., Girault, J.P., Igolen, J., Chottard, J.C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antineoplastic agents Base Composition Base Sequence Biological and medical sciences Cisplatin - chemistry DNA - chemistry General aspects Magnetic Resonance Spectroscopy - methods Medical sciences Molecular Sequence Data Oligodeoxyribonucleotides - chemical synthesis Oligodeoxyribonucleotides - chemistry Organoplatinum Compounds - chemical synthesis Organoplatinum Compounds - chemistry Pharmacology. Drug treatments Spectrophotometry, Ultraviolet
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Beschreibung
Zusammenfassung:	The nonamer 5′d(CTCAGCCTC) 3′ 1 has been reacted with cis-diamminediaquaplatinum(II) in water at pH 4.2. The major reaction product was shown by enzymatic digestion and 1H NMR to be the d(ApG) cis-Pt(NH 3) 2 chelate [ cis-Pt(NH 3) 2{d(CTCAGCCTC)- N7(4), N7(5)}] 1-Pt. When mixed with its complementary strand 2, 1-Pt forms a B DNA type duplex 3-Pt with a Tm of 35°C (versus 58°C for the unplatinated duplex). The NMR study of the exchangeable protons of 3-Pt revealed that the helix distortion is localized on the CA ★G ★-CTG moiety (the asterisks indicating the platinum chelation sites) with a strong perturbation of the A ★(4)T(15) base pair related to a large tilt of A ★(4).
ISSN:	0006-291X 1090-2104
DOI:	10.1016/0006-291X(92)91768-L