One-step transformation of yeast in stationary phase

A fast yeast-transformation technique has been developed by adding thio compounds to alkali-ion based protocols and incubating at 45 degrees C. This procedure is especially recommended for cells from stationary phase at a density up to 2.5 x 10(8) cells/ml. It involves only one step for the preparat...

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Veröffentlicht in:	Current genetics 1992, Vol.21 (1), p.83-84
Hauptverfasser:	Chen, D C, Yang, B C, Kuo, T T
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Classical genetics, quantitative genetics, hybrids DNA, Fungal - genetics Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Schizosaccharomyces - genetics Thallophyta, bryophyta Transformation, Genetic Vegetals Yeasts - genetics Yeasts - growth & development
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Beschreibung
Zusammenfassung:	A fast yeast-transformation technique has been developed by adding thio compounds to alkali-ion based protocols and incubating at 45 degrees C. This procedure is especially recommended for cells from stationary phase at a density up to 2.5 x 10(8) cells/ml. It involves only one step for the preparation and transformation of competent cells within 30 min. The yield was more than 10(4) transformants/microgram plasmid DNA. This protocol is easy to scale up for many DNA samples and is also applicable for yeast cells from different types of storages.
ISSN:	0172-8083 1432-0983
DOI:	10.1007/BF00318659