Frame-shift mutations within the vaccinia virus A-type inclusion protein gene
The genetic basis for the failure of vaccinia virus (strain WR) to forma full-length 150 kiloDalton (kDa) A-type inclusion protein was determined by sequencing a 4.1-kb pair segment of DNA and analyzing its transcription products. Open reading frames predicted to encode slightly overlapping 84.5- an...
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Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1992-02, Vol.186 (2), p.777-782 |
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Sprache: | eng |
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Zusammenfassung: | The genetic basis for the failure of vaccinia virus (strain WR) to forma full-length 150 kiloDalton (kDa) A-type inclusion protein was determined by sequencing a 4.1-kb pair segment of DNA and analyzing its transcription products. Open reading frames predicted to encode slightly overlapping 84.5- and 27.1-kDa proteins homologous to contiguous N-terminal segments of the A-type inclusion protein of cowpox virus were found. A putative deletion of two adjacent nucleotides occurring within several consecutive AG repeats and an insertion of 8 nucleotides accounted for the first and second reading frame shifts, respectively. Additional small mutations affecting reading frames were present in the C-terminal region of the gene. The vaccinia and cowpox virus mRNAs encoding the disparate size A-type inclusion proteins were similar in length, had equivalent 5′ and 3′ ends, and were expressed late in infection indicating the absence of mutations affecting transcriptional signals. |
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ISSN: | 0042-6822 1096-0341 |
DOI: | 10.1016/0042-6822(92)90046-R |