The Adrenaline Test for Enzymes

The Adrenaline Test for Enzymes

A versatile high‐throughput enzyme assay is demonstrated which is based on the colorimetric back‐titration of sodium periodate with L‐adrenaline (1). Enzyme activity is associated with the depletion of sodium periodate by the reaction product (P), and indicated by the decrease in the amount of the r...

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Veröffentlicht in:Angewandte Chemie International Edition 2002-04, Vol.41 (7), p.1229-1232
Hauptverfasser: Wahler, Denis, Reymond, Jean-Louis
Format: Artikel
Sprache:eng
Schlagworte:
Combinatorial Chemistry Techniques - methods
enzyme assays
Enzymes - chemistry
Enzymes - metabolism
Epinephrine - chemistry
high-throughput screening
hydrolases
lipases
phytases
Substrate Specificity
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Zusammenfassung:A versatile high‐throughput enzyme assay is demonstrated which is based on the colorimetric back‐titration of sodium periodate with L‐adrenaline (1). Enzyme activity is associated with the depletion of sodium periodate by the reaction product (P), and indicated by the decrease in the amount of the red dye adrenochrome (2) produced by the oxidation of adrenaline by sodium periodate.
ISSN:1433-7851
1521-3773
DOI:10.1002/1521-3773(20020402)41:7<1229::AID-ANIE1229>3.0.CO;2-5