PCR cloning of the full-length cDNA for the seed protein canavalin from the jack bean plant, Canavalis ensiformis

To obtain detailed genetic and structural information on the reverse proteins of legumes, a full-length cDNA for canavalin was generated by use of the polymerase chain reaction (PCR). A single-stranded cDNA template synthesized from total jack bean RNA by reverse transcriptase was used with primer pairs selected from the 5' and 3' sequence of the cDNA coding for the vicilin protein from Canavalia gladiata .