Identification and map position of YAC clones comprising one‐third of the Arabidopsis genome

Identification and map position of YAC clones comprising one‐third of the Arabidopsis genome

Summary YAC clones corresponding to 125 Arabidopsis thaliana RFLP markers have been identified. At least one YAC clone has been isolated for each of the RFLP markers tested. Based on CHEF gel analysis of 196 clones, the mean insert size of the available Arabidopsis YAC libraries is approximately 160...

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Veröffentlicht in:The Plant journal : for cell and molecular biology 1991-11, Vol.1 (3), p.367-374
Hauptverfasser: Hwang, I, Kohchi, T, Hauge, BM, Goodman, HM, Schmidt, R, Cnops, G, Dean, C, Gibson, S, Iba, K, Lemieux, B
Format: Artikel
Sprache:eng
Schlagworte:
Arabidopsis - genetics
Arabidopsis thaliana
Chromosome Mapping
Cloning, Molecular
Genes, Plant
Genome
Plants - genetics
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Zusammenfassung:Summary YAC clones corresponding to 125 Arabidopsis thaliana RFLP markers have been identified. At least one YAC clone has been isolated for each of the RFLP markers tested. Based on CHEF gel analysis of 196 clones, the mean insert size of the available Arabidopsis YAC libraries is approximately 160 kb. The YACs of known genetic map location encompass about 30% of the Arabidopsis genome. The results presented here represent a first step towards assembly of an overlapping YAC library of the A. thaliana genome.
ISSN:0960-7412
1365-313X
DOI:10.1046/j.1365-313X.1991.t01-5-00999.x