CHARACTERIZATION OF ANTI-Leb ANTIBODY PURIFIED FROM SERUM OF IMMUNIZED RABBITS

An anti-Leb antibody was produced in sera of rabbits by immunization with human saliva from blood group O Le (a-b+) secretor and purified by sequential use of silica beads immobilized with H type 1, Lea and Leb. The purified antibody agglutinated only Le (a-b+) red cells irrespective of their ABO blood type. Hemagglutination reaction with the antibody of blood group O Le (a-b+) red cells was inhibited not only by saliva samples from blood group Le (a-b+) secretors and Synsorb beads immobilized with Leb hapten, but also weakly by Synsorb immobilized with Y and H type 2 haptens.