The apoptotic effect of somatostatin analogue SMS 201-995 on human lymphocytes
The antiproliferative effect of a synthetic octapeptide, somatostatin analogue SMS 201-995 (SMS), and its capacity to bind were evaluated on human peripheral blood lymphocytes (PBL) activated by phytohemoagglutinin (PHA). We then addressed our work to investigate if SMS inhibits PHA activation of PB...
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| Veröffentlicht in: | Journal of neuroimmunology 2002-12, Vol.133 (1), p.211-216 |
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| creator | Lattuada, D Casnici, C Venuto, A Marelli, O |
| description | The antiproliferative effect of a synthetic octapeptide, somatostatin analogue SMS 201-995 (SMS), and its capacity to bind were evaluated on human peripheral blood lymphocytes (PBL) activated by phytohemoagglutinin (PHA).
We then addressed our work to investigate if SMS inhibits PHA activation of PBL by a cytostatic rather than a cytotoxic mechanism. Consequently, we studied the cell cycle distribution and the activation of caspase-3, measuring the presence of the cleavage product of poly(ADP-ribose) polymerases (PARP), and we evaluated the presence of apoptotic DNA by using a monoclonal antibody specific for the single-stranded regions of DNA. All our results indicate that SMS induces apoptosis in activated lymphocytes. |
| doi_str_mv | 10.1016/S0165-5728(02)00364-8 |
| format | Article |
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We then addressed our work to investigate if SMS inhibits PHA activation of PBL by a cytostatic rather than a cytotoxic mechanism. Consequently, we studied the cell cycle distribution and the activation of caspase-3, measuring the presence of the cleavage product of poly(ADP-ribose) polymerases (PARP), and we evaluated the presence of apoptotic DNA by using a monoclonal antibody specific for the single-stranded regions of DNA. All our results indicate that SMS induces apoptosis in activated lymphocytes.</description><subject>Adult</subject><subject>Anti-Inflammatory Agents - pharmacology</subject><subject>Anti-Inflammatory Agents - therapeutic use</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Apoptosis - immunology</subject><subject>Caspase 3</subject><subject>Caspases - drug effects</subject><subject>Caspases - immunology</subject><subject>Cell Cycle - drug effects</subject><subject>Cell Cycle - immunology</subject><subject>Cells, Cultured</subject><subject>Chemotaxis, Leukocyte - drug effects</subject><subject>Chemotaxis, Leukocyte - immunology</subject><subject>DNA - drug effects</subject><subject>Human lymphocytes</subject><subject>Humans</subject><subject>Immunosuppressive Agents - pharmacology</subject><subject>Immunosuppressive Agents - therapeutic use</subject><subject>Lymphocytes - drug effects</subject><subject>Lymphocytes - immunology</subject><subject>Middle Aged</subject><subject>Octreotide</subject><subject>Octreotide - pharmacology</subject><subject>Octreotide - therapeutic use</subject><subject>Proteins - drug effects</subject><subject>Proteins - immunology</subject><subject>Somatostatin</subject><subject>Somatostatin - analogs & derivatives</subject><issn>0165-5728</issn><issn>1872-8421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1P3DAURa2qqEwHfkIrryq6CPg9x_lYVdWIFqQBFgNry3GeO6mSOI2dSvPvCcwIlrN5b3PuvdJh7AuISxCQXW3moxKVY3Eh8LsQMkuT4gNbQJFjUqQIH9niDTlln0P4KwQomZaf2ClgmmYC1YLdP26Jm8EP0cfGcnKObOTe8eA7E32IJjY9N71p_Z-J-OZuw1FAUpaK-55vp870vN11w9bbXaRwxk6caQOdH_6SPf26flzdJOuH37ern-vEyhJjkoOroJZCYgY1lCWBFOgyQKizmSBZyUI6gWlFoHJIrSBrK1m7DKXNKimX7Nu-dxj9v4lC1F0TLLWt6clPQeeYy1wqPApCocpcIcyg2oN29CGM5PQwNp0ZdxqEfjGuX43rF51aoH41ros59_UwMFUd1e-pg-IZ-LEHaPbxv6FRB9tQb6luxtm1rn1zZOIZmoeOJA</recordid><startdate>20021201</startdate><enddate>20021201</enddate><creator>Lattuada, D</creator><creator>Casnici, C</creator><creator>Venuto, A</creator><creator>Marelli, O</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TK</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20021201</creationdate><title>The apoptotic effect of somatostatin analogue SMS 201-995 on human lymphocytes</title><author>Lattuada, D ; Casnici, C ; Venuto, A ; Marelli, O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-71fb1d303261d199e1302f6121d6c39e3b383f024be15714c0eccb3df623c6b33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adult</topic><topic>Anti-Inflammatory Agents - pharmacology</topic><topic>Anti-Inflammatory Agents - therapeutic use</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Apoptosis - immunology</topic><topic>Caspase 3</topic><topic>Caspases - drug effects</topic><topic>Caspases - immunology</topic><topic>Cell Cycle - drug effects</topic><topic>Cell Cycle - immunology</topic><topic>Cells, Cultured</topic><topic>Chemotaxis, Leukocyte - drug effects</topic><topic>Chemotaxis, Leukocyte - immunology</topic><topic>DNA - drug effects</topic><topic>Human lymphocytes</topic><topic>Humans</topic><topic>Immunosuppressive Agents - pharmacology</topic><topic>Immunosuppressive Agents - therapeutic use</topic><topic>Lymphocytes - drug effects</topic><topic>Lymphocytes - immunology</topic><topic>Middle Aged</topic><topic>Octreotide</topic><topic>Octreotide - pharmacology</topic><topic>Octreotide - therapeutic use</topic><topic>Proteins - drug effects</topic><topic>Proteins - immunology</topic><topic>Somatostatin</topic><topic>Somatostatin - analogs & derivatives</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lattuada, D</creatorcontrib><creatorcontrib>Casnici, C</creatorcontrib><creatorcontrib>Venuto, A</creatorcontrib><creatorcontrib>Marelli, O</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroimmunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lattuada, D</au><au>Casnici, C</au><au>Venuto, A</au><au>Marelli, O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The apoptotic effect of somatostatin analogue SMS 201-995 on human lymphocytes</atitle><jtitle>Journal of neuroimmunology</jtitle><addtitle>J Neuroimmunol</addtitle><date>2002-12-01</date><risdate>2002</risdate><volume>133</volume><issue>1</issue><spage>211</spage><epage>216</epage><pages>211-216</pages><issn>0165-5728</issn><eissn>1872-8421</eissn><abstract>The antiproliferative effect of a synthetic octapeptide, somatostatin analogue SMS 201-995 (SMS), and its capacity to bind were evaluated on human peripheral blood lymphocytes (PBL) activated by phytohemoagglutinin (PHA).
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adult Anti-Inflammatory Agents - pharmacology Anti-Inflammatory Agents - therapeutic use Apoptosis Apoptosis - drug effects Apoptosis - immunology Caspase 3 Caspases - drug effects Caspases - immunology Cell Cycle - drug effects Cell Cycle - immunology Cells, Cultured Chemotaxis, Leukocyte - drug effects Chemotaxis, Leukocyte - immunology DNA - drug effects Human lymphocytes Humans Immunosuppressive Agents - pharmacology Immunosuppressive Agents - therapeutic use Lymphocytes - drug effects Lymphocytes - immunology Middle Aged Octreotide Octreotide - pharmacology Octreotide - therapeutic use Proteins - drug effects Proteins - immunology Somatostatin Somatostatin - analogs & derivatives |
| title | The apoptotic effect of somatostatin analogue SMS 201-995 on human lymphocytes |
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