The apoptotic effect of somatostatin analogue SMS 201-995 on human lymphocytes

The antiproliferative effect of a synthetic octapeptide, somatostatin analogue SMS 201-995 (SMS), and its capacity to bind were evaluated on human peripheral blood lymphocytes (PBL) activated by phytohemoagglutinin (PHA). We then addressed our work to investigate if SMS inhibits PHA activation of PB...

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Veröffentlicht in:	Journal of neuroimmunology 2002-12, Vol.133 (1), p.211-216
Hauptverfasser:	Lattuada, D, Casnici, C, Venuto, A, Marelli, O
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Anti-Inflammatory Agents - pharmacology Anti-Inflammatory Agents - therapeutic use Apoptosis Apoptosis - drug effects Apoptosis - immunology Caspase 3 Caspases - drug effects Caspases - immunology Cell Cycle - drug effects Cell Cycle - immunology Cells, Cultured Chemotaxis, Leukocyte - drug effects Chemotaxis, Leukocyte - immunology DNA - drug effects Human lymphocytes Humans Immunosuppressive Agents - pharmacology Immunosuppressive Agents - therapeutic use Lymphocytes - drug effects Lymphocytes - immunology Middle Aged Octreotide Octreotide - pharmacology Octreotide - therapeutic use Proteins - drug effects Proteins - immunology Somatostatin Somatostatin - analogs & derivatives
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Zusammenfassung:	The antiproliferative effect of a synthetic octapeptide, somatostatin analogue SMS 201-995 (SMS), and its capacity to bind were evaluated on human peripheral blood lymphocytes (PBL) activated by phytohemoagglutinin (PHA). We then addressed our work to investigate if SMS inhibits PHA activation of PBL by a cytostatic rather than a cytotoxic mechanism. Consequently, we studied the cell cycle distribution and the activation of caspase-3, measuring the presence of the cleavage product of poly(ADP-ribose) polymerases (PARP), and we evaluated the presence of apoptotic DNA by using a monoclonal antibody specific for the single-stranded regions of DNA. All our results indicate that SMS induces apoptosis in activated lymphocytes.
ISSN:	0165-5728 1872-8421
DOI:	10.1016/S0165-5728(02)00364-8