Saponins, IL12 and BCG adjuvant in the FML-vaccine formulation against murine visceral leishmaniasis

Saponins, IL12 and BCG adjuvant in the FML-vaccine formulation against murine visceral leishmaniasis

The FML antigen of Leishmania donovani, in combination with either Riedel de Haën (R), QuilA, QS21 saponins, IL12 or BCG, was used in vaccination of an outbred murine model against visceral leishmaniasis (VL). Significant and specific increases in anti-FML IgG and IgM responses were detected for all...

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Veröffentlicht in:Vaccine 2002-11, Vol.21 (1), p.30-43
Hauptverfasser: Santos, Wania Renata, de Lima, Valeria M.F, de Souza, Edilma Paraguai, Bernardo, Robson Ronney, Palatnik, Marcos, de Sousa, Clarisa Beatriz Palatnik
Format: Artikel
Sprache:eng
Schlagworte:
Adjuvants
Adjuvants, Immunologic - administration & dosage
Animals
Antigens, Protozoan - immunology
Biological and medical sciences
Human protozoal diseases
IL12
Infectious diseases
Interleukin-12 - therapeutic use
Leishmania donovani - immunology
Leishmaniasis, Visceral - prevention & control
Leshmaniasis
Liver - parasitology
Medical sciences
Mice
Murine visceral leishmaniasis
Mycobacterium bovis
Parasitic diseases
Protozoal diseases
Protozoan Vaccines - administration & dosage
Protozoan Vaccines - immunology
QS21
QuilA
Saponin
Saponins - therapeutic use
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Zusammenfassung:The FML antigen of Leishmania donovani, in combination with either Riedel de Haën (R), QuilA, QS21 saponins, IL12 or BCG, was used in vaccination of an outbred murine model against visceral leishmaniasis (VL). Significant and specific increases in anti-FML IgG and IgM responses were detected for all adjuvants, and in anti-FML IgG1, IgG2a and IgG2b and delayed type of hypersensitivity to L. donovani lysate (DTH), only for all saponins and IL12. The QS21-FML and QuilA-FML groups achieved the highest IgG2a response. QuilA-FML developed the strongest DTH and QS21-FML animals showed the highest serum IFN-γ concentrations. The reduction of parasitic load in the liver in response to each FML-vaccine formulation was: 52% ( P
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(02)00444-9