Organic disulfides as a means to generate streak-free two-dimensional maps with narrow range basic immobilized pH gradient strips as first dimension

Organic disulfides as a means to generate streak-free two-dimensional maps with narrow range basic immobilized pH gradient strips as first dimension

Streaking is a severe problem when narrow range basic immobilized pH gradient strips are used as the first dimension of two‐dimensional (2‐D) electrophoresis. It is demonstrated that this cysteinyl related streaking is eliminated when focusing is done in the presence of hydroxyethyl disulfide (DeStr...

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Veröffentlicht in:Proteomics (Weinheim) 2002-11, Vol.2 (11), p.1630-1632
Hauptverfasser: Olsson, Ingmar, Larsson, Kjell, Palmgren, Ronnie, Bjellqvist, Bengt
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Biological and medical sciences
Biotechnology
Disulfides - chemistry
Electrophoresis, Gel, Two-Dimensional - methods
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
Immobilization of enzymes and other molecules
Immobilization techniques
Liver - chemistry
Methods. Procedures. Technologies
Mice
Miscellaneous
Molecular and cellular biology
Molecular genetics
Proteome - analysis
Proteome - chemistry
Proteomics - methods
Two-dimensional electrophoresis
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Zusammenfassung:Streaking is a severe problem when narrow range basic immobilized pH gradient strips are used as the first dimension of two‐dimensional (2‐D) electrophoresis. It is demonstrated that this cysteinyl related streaking is eliminated when focusing is done in the presence of hydroxyethyl disulfide (DeStreak™). Use of DeStreak also results in 2‐D maps with simplified spot patterns and improved reproducibility.
ISSN:1615-9853
1615-9861
DOI:10.1002/1615-9861(200211)2:11<1630::AID-PROT1630>3.0.CO;2-N