Claudin-2 expression induces cation-selective channels in tight junctions of epithelial cells

Tight junctions seal the paracellular pathway of epithelia but, in leaky tissues, also exhibit specific permeability. In order to characterize the contribution of claudin-2 to barrier and permeability properties of the tight junction in detail, we studied two strains of Madin-Darby canine kidney cel...

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Veröffentlicht in:	Journal of cell science 2002-12, Vol.115 (Pt 24), p.4969-4976
Hauptverfasser:	Amasheh, Salah, Meiri, Noga, Gitter, Alfred H, Schöneberg, Torsten, Mankertz, Joachim, Schulzke, Jörg D, Fromm, Michael
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Cations Cell Line Claudins DNA Primers Dogs Fluorescent Antibody Technique Ion Channels - biosynthesis Membrane Proteins - genetics Mice Transfection
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Zusammenfassung:	Tight junctions seal the paracellular pathway of epithelia but, in leaky tissues, also exhibit specific permeability. In order to characterize the contribution of claudin-2 to barrier and permeability properties of the tight junction in detail, we studied two strains of Madin-Darby canine kidney cells (MDCK-C7 and MDCK-C11) with different tight junctional permeabilities. Monolayers of C7 cells exhibited a high transepithelial resistance (>1 kOhms cm(2)), compared with C11 cells (
ISSN:	0021-9533 1477-9137
DOI:	10.1242/jcs.00165