The alpha C protein mediates internalization of group B Streptococcus within human cervical epithelial cells
Summary Group B Streptococcus (GBS) is the leading cause of bacterial chorioamnionitis and neonatal pneumonia, sepsis, and meningitis. Deletion of the alpha C protein gene (bca) attenuates the virulence of GBS in an animal model; significant survival differences in the first 24 h of infection sugges...
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Veröffentlicht in: | Cellular microbiology 2002-11, Vol.4 (11), p.751-758 |
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Sprache: | eng |
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Group B Streptococcus (GBS) is the leading cause of bacterial chorioamnionitis and neonatal pneumonia, sepsis, and meningitis. Deletion of the alpha C protein gene (bca) attenuates the virulence of GBS in an animal model; significant survival differences in the first 24 h of infection suggest a pathogenic role for the alpha C protein early in the infection process. We examined the role of alpha C protein in the association between GBS and mucosal surfaces using a human cervical epithelial cell line, ME180. Fluorescent and confocal microscopy and flow cytometry demonstrated that 9‐repeat alpha C protein binds to the surface of ME180 cells. Isolated N‐terminal region of this protein also binds to these cells and competitively inhibits binding of the full protein. Wild‐type GBS strain A909 and the bca‐null isogenic mutant JL2053 bound similarly to the surface of ME180 cells. However, A909 entered these cells threefold more. Internalization of A909 was inhibited with 2‐ and 9‐repeat alpha C and with N‐terminal region alone but not by repeat region‐specific peptide. Translocation across polarized ME180 membranes was fivefold greater for A909 than for JL2053. These findings suggest a role for the alpha C protein in interaction with epithelial surfaces and initiation of infection. |
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ISSN: | 1462-5814 1462-5822 |
DOI: | 10.1046/j.1462-5822.2002.00227.x |