VACUOLE FORMATION IN MAST CELLS RESPONDING TO OSMOTIC STRESS AND TO F-ACTIN DISASSEMBLY

Fluorescent probes were used to visualize the morphology of membranes and of F-actin in rat peritoneal mast cells, exposed to hyperosmotic medium and consequently reversed to isotonicity. Hypertonicity induced cell shrinkage followed by a regulatory volume increase, and cell alkalinization that was...

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Veröffentlicht in:Cell biology international 2002-01, Vol.26 (10), p.885-892
Hauptverfasser: Koffer, Anna, Williams, Mark, Johansen, Torben
Format: Artikel
Sprache:eng
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Zusammenfassung:Fluorescent probes were used to visualize the morphology of membranes and of F-actin in rat peritoneal mast cells, exposed to hyperosmotic medium and consequently reversed to isotonicity. Hypertonicity induced cell shrinkage followed by a regulatory volume increase, and cell alkalinization that was sensitive to amiloride, an inhibitor of the Na +/H + exchanger (NHE), but not to Latrunculin B, an inhibitor of actin polymerization. Using Bodipy-Sphingomyelin, we have observed formation of vacuole-like dilations (VLDs), primarily at or close to the adhesion plane, following the reversal from hyper- to isotonic medium. VLD formation was not inhibited by Latrunculin B or by amiloride. Phalloidin staining has shown that actin filaments do not surround the vacuoles and latrunculin-induced depolymerization of actin has actually promoted vacuole formation, even in isotonic conditions. The results support the idea that a decrease in membrane tension promotes the internalization of the plasma membrane.
ISSN:1065-6995
1095-8355
DOI:10.1006/cbir.2002.0940