Group II introns: highly specific endonucleases with modular structures and diverse catalytic functions

Group II introns: highly specific endonucleases with modular structures and diverse catalytic functions

Group II introns are large catalytic RNAs with a remarkable repertoire of reactions. Here we present construct designs and protocols that were used to develop a set of kinetic frameworks for studying the structure and reaction mechanisms of group II introns and ribozymes derived from them. In additi...

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Veröffentlicht in:Methods (San Diego, Calif.) Calif.), 2002-11, Vol.28 (3), p.323-335
Hauptverfasser: Fedorova, Olga, Julie Su, Linhui, Pyle, Anna Marie
Format: Artikel
Sprache:eng
Schlagworte:
Catalysis
Group II intron
Hydrolysis
Introns
Kinetics
Nucleic Acid Conformation
Ribozyme
RNA Splicing
RNA, Catalytic - chemistry
RNA, Catalytic - physiology
Self-splicing
Structure-Activity Relationship
Substrate Specificity
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Zusammenfassung:Group II introns are large catalytic RNAs with a remarkable repertoire of reactions. Here we present construct designs and protocols that were used to develop a set of kinetic frameworks for studying the structure and reaction mechanisms of group II introns and ribozymes derived from them. In addition, we discuss application of these systems to structure/function analysis of the ai5γ group II intron.
ISSN:1046-2023
1095-9130
DOI:10.1016/S1046-2023(02)00239-6