Comparison of tissue culture and animal models for assessment of Cryptospridium parvum infection
The current increased interest for using tissue culture as a surrogate for mouse infection to assess Cryptospridium viability suggests that a comparison of the two models is essential for data interpretation. Therefore, a need remains for a statistical comparison that can demonstrate if infection an...
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| Veröffentlicht in: | Experimental parasitology 2002-06, Vol.101 (2), p.97-106 |
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| creator | Slifko, Theresa R Huffman, Debra E Dussert, Bertrand Owens, James H Jakubowski, Walter Haas, Charles N Rose, Joan B |
| description | The current increased interest for using tissue culture as a surrogate for mouse infection to assess
Cryptospridium viability suggests that a comparison of the two models is essential for data interpretation. Therefore, a need remains for a statistical comparison that can demonstrate if infection and inactivation predicted by new tissue culture models are comparable with those predicted by animal models. Data from a total of 31 dose–response trials using both tissue culture and mouse models to assess
C. parvum infectivity were compared. The dose needed to infect 50% of the tissue cultures (ID
50) was also compared to each ID
50 in mice. Average ID
50s developed using the logit dose–response method for tissue culture and mice were 8 and 107, respectively, suggesting that tissue culture was more sensitive to infection. However, correlation (
r) between tissue culture and mouse infectivity was statistically significant (0.9167 [95% CI=0.8428 to 0.9594,
p |
| doi_str_mv | 10.1016/S0014-4894(02)00100-5 |
| format | Article |
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Cryptospridium viability suggests that a comparison of the two models is essential for data interpretation. Therefore, a need remains for a statistical comparison that can demonstrate if infection and inactivation predicted by new tissue culture models are comparable with those predicted by animal models. Data from a total of 31 dose–response trials using both tissue culture and mouse models to assess
C. parvum infectivity were compared. The dose needed to infect 50% of the tissue cultures (ID
50) was also compared to each ID
50 in mice. Average ID
50s developed using the logit dose–response method for tissue culture and mice were 8 and 107, respectively, suggesting that tissue culture was more sensitive to infection. However, correlation (
r) between tissue culture and mouse infectivity was statistically significant (0.9167 [95% CI=0.8428 to 0.9594,
p<0.0001]). Comparison of oocyst disinfection by UV and chlorine dioxide showed no significant difference between inactivation predicted by tissue culture and mouse models (
p=0.8893;
t=0.0141;
n=21). These results demonstrate that tissue culture can successfully be used to measure
C. parvum infection and can be used for determining inactivation in disinfection studies.</description><identifier>ISSN: 0014-4894</identifier><identifier>EISSN: 1090-2449</identifier><identifier>DOI: 10.1016/S0014-4894(02)00100-5</identifier><identifier>PMID: 12427463</identifier><identifier>CODEN: EXPAAA</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Adenocarcinoma ; Animals ; Animals, Newborn ; Biological and medical sciences ; ClO 2 ; Correlation ; Cryptosporidiosis - parasitology ; Cryptosporidium parvum - physiology ; Cryptospridium parvum ; Disease Models, Animal ; Fundamental and applied biological sciences. Psychology ; HCT-8 cell line ; Humans ; Ileal Neoplasms ; Ileocecal Valve ; Inactivation ; Life cycle. Host-agent relationship. Pathogenesis ; Mice ; Mice, Inbred BALB C ; Mouse infectivity ; Protozoa ; Regression Analysis ; Tissue culture ; Tumor Cells, Cultured ; Zoonotic</subject><ispartof>Experimental parasitology, 2002-06, Vol.101 (2), p.97-106</ispartof><rights>2002 Elsevier Science (USA)</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-2fb67d8a92a221f38d16eb6f1ed997122a1c948e79e6d524eff275a54f0eede23</citedby><cites>FETCH-LOGICAL-c391t-2fb67d8a92a221f38d16eb6f1ed997122a1c948e79e6d524eff275a54f0eede23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0014-4894(02)00100-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14353058$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12427463$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Slifko, Theresa R</creatorcontrib><creatorcontrib>Huffman, Debra E</creatorcontrib><creatorcontrib>Dussert, Bertrand</creatorcontrib><creatorcontrib>Owens, James H</creatorcontrib><creatorcontrib>Jakubowski, Walter</creatorcontrib><creatorcontrib>Haas, Charles N</creatorcontrib><creatorcontrib>Rose, Joan B</creatorcontrib><title>Comparison of tissue culture and animal models for assessment of Cryptospridium parvum infection</title><title>Experimental parasitology</title><addtitle>Exp Parasitol</addtitle><description>The current increased interest for using tissue culture as a surrogate for mouse infection to assess
Cryptospridium viability suggests that a comparison of the two models is essential for data interpretation. Therefore, a need remains for a statistical comparison that can demonstrate if infection and inactivation predicted by new tissue culture models are comparable with those predicted by animal models. Data from a total of 31 dose–response trials using both tissue culture and mouse models to assess
C. parvum infectivity were compared. The dose needed to infect 50% of the tissue cultures (ID
50) was also compared to each ID
50 in mice. Average ID
50s developed using the logit dose–response method for tissue culture and mice were 8 and 107, respectively, suggesting that tissue culture was more sensitive to infection. However, correlation (
r) between tissue culture and mouse infectivity was statistically significant (0.9167 [95% CI=0.8428 to 0.9594,
p<0.0001]). Comparison of oocyst disinfection by UV and chlorine dioxide showed no significant difference between inactivation predicted by tissue culture and mouse models (
p=0.8893;
t=0.0141;
n=21). These results demonstrate that tissue culture can successfully be used to measure
C. parvum infection and can be used for determining inactivation in disinfection studies.</description><subject>Adenocarcinoma</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Biological and medical sciences</subject><subject>ClO 2</subject><subject>Correlation</subject><subject>Cryptosporidiosis - parasitology</subject><subject>Cryptosporidium parvum - physiology</subject><subject>Cryptospridium parvum</subject><subject>Disease Models, Animal</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HCT-8 cell line</subject><subject>Humans</subject><subject>Ileal Neoplasms</subject><subject>Ileocecal Valve</subject><subject>Inactivation</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mouse infectivity</subject><subject>Protozoa</subject><subject>Regression Analysis</subject><subject>Tissue culture</subject><subject>Tumor Cells, Cultured</subject><subject>Zoonotic</subject><issn>0014-4894</issn><issn>1090-2449</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMlKBDEQhoMoOi6PoPRF0UNrkk4vOYkMbjDgQT3HTFKBSHdnTHUP-PZmFvTooaoIfH-l-Ag5ZfSaUVbdvFLKRC4aKS4pv0oPSvNyh0wYlTTnQshdMvlFDsgh4ieltGFc7JOD1HktqmJCPqahW-joMfRZcNngEUfIzNgOY4RM9zaV73SbdcFCi5kLMdOIgNhBP6wi0_i9GAIuord-7LK0bJmG7x2YwYf-mOw53SKcbOcReX-4f5s-5bOXx-fp3Sw3hWRDzt28qm2jJdecM1c0llUwrxwDK2XNONfMSNFALaGyJRfgHK9LXQpHASzw4ohcbPYuYvgaAQfVeTTQtrqHMKKqeVUnASyB5QY0MSBGcCqd3un4rRhVK7VqrVatvCnK1VqtKlPubPvBOO_A_qW2LhNwvgU0Gt26qHvj8Y8TRVnQsknc7YZLPmHpISo0HnoD1sfkTNng_znlB7pzlyk</recordid><startdate>20020601</startdate><enddate>20020601</enddate><creator>Slifko, Theresa R</creator><creator>Huffman, Debra E</creator><creator>Dussert, Bertrand</creator><creator>Owens, James H</creator><creator>Jakubowski, Walter</creator><creator>Haas, Charles N</creator><creator>Rose, Joan B</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020601</creationdate><title>Comparison of tissue culture and animal models for assessment of Cryptospridium parvum infection</title><author>Slifko, Theresa R ; Huffman, Debra E ; Dussert, Bertrand ; Owens, James H ; Jakubowski, Walter ; Haas, Charles N ; Rose, Joan B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-2fb67d8a92a221f38d16eb6f1ed997122a1c948e79e6d524eff275a54f0eede23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adenocarcinoma</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Biological and medical sciences</topic><topic>ClO 2</topic><topic>Correlation</topic><topic>Cryptosporidiosis - parasitology</topic><topic>Cryptosporidium parvum - physiology</topic><topic>Cryptospridium parvum</topic><topic>Disease Models, Animal</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HCT-8 cell line</topic><topic>Humans</topic><topic>Ileal Neoplasms</topic><topic>Ileocecal Valve</topic><topic>Inactivation</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mouse infectivity</topic><topic>Protozoa</topic><topic>Regression Analysis</topic><topic>Tissue culture</topic><topic>Tumor Cells, Cultured</topic><topic>Zoonotic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Slifko, Theresa R</creatorcontrib><creatorcontrib>Huffman, Debra E</creatorcontrib><creatorcontrib>Dussert, Bertrand</creatorcontrib><creatorcontrib>Owens, James H</creatorcontrib><creatorcontrib>Jakubowski, Walter</creatorcontrib><creatorcontrib>Haas, Charles N</creatorcontrib><creatorcontrib>Rose, Joan B</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Slifko, Theresa R</au><au>Huffman, Debra E</au><au>Dussert, Bertrand</au><au>Owens, James H</au><au>Jakubowski, Walter</au><au>Haas, Charles N</au><au>Rose, Joan B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of tissue culture and animal models for assessment of Cryptospridium parvum infection</atitle><jtitle>Experimental parasitology</jtitle><addtitle>Exp Parasitol</addtitle><date>2002-06-01</date><risdate>2002</risdate><volume>101</volume><issue>2</issue><spage>97</spage><epage>106</epage><pages>97-106</pages><issn>0014-4894</issn><eissn>1090-2449</eissn><coden>EXPAAA</coden><abstract>The current increased interest for using tissue culture as a surrogate for mouse infection to assess
Cryptospridium viability suggests that a comparison of the two models is essential for data interpretation. Therefore, a need remains for a statistical comparison that can demonstrate if infection and inactivation predicted by new tissue culture models are comparable with those predicted by animal models. Data from a total of 31 dose–response trials using both tissue culture and mouse models to assess
C. parvum infectivity were compared. The dose needed to infect 50% of the tissue cultures (ID
50) was also compared to each ID
50 in mice. Average ID
50s developed using the logit dose–response method for tissue culture and mice were 8 and 107, respectively, suggesting that tissue culture was more sensitive to infection. However, correlation (
r) between tissue culture and mouse infectivity was statistically significant (0.9167 [95% CI=0.8428 to 0.9594,
p<0.0001]). Comparison of oocyst disinfection by UV and chlorine dioxide showed no significant difference between inactivation predicted by tissue culture and mouse models (
p=0.8893;
t=0.0141;
n=21). These results demonstrate that tissue culture can successfully be used to measure
C. parvum infection and can be used for determining inactivation in disinfection studies.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>12427463</pmid><doi>10.1016/S0014-4894(02)00100-5</doi><tpages>10</tpages></addata></record> |
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| subjects | Adenocarcinoma Animals Animals, Newborn Biological and medical sciences ClO 2 Correlation Cryptosporidiosis - parasitology Cryptosporidium parvum - physiology Cryptospridium parvum Disease Models, Animal Fundamental and applied biological sciences. Psychology HCT-8 cell line Humans Ileal Neoplasms Ileocecal Valve Inactivation Life cycle. Host-agent relationship. Pathogenesis Mice Mice, Inbred BALB C Mouse infectivity Protozoa Regression Analysis Tissue culture Tumor Cells, Cultured Zoonotic |
| title | Comparison of tissue culture and animal models for assessment of Cryptospridium parvum infection |
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