Physical and genetic map of the Neisseria gonorrhoeae strain MS11‐N198 chromosome

Summary A macro‐restriction map of the Neisseria gonorrhoeae chromosome was constructed using the enzymes Nhel and Spel. Combinations of one‐and two‐dimensional electrophoresis of completely or partially digested chromosomal DNA were performed to align the restriction fragments. The chromosome is ci...

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Veröffentlicht in:	Molecular microbiology 1991-10, Vol.5 (10), p.2529-2539
Hauptverfasser:	Bihlmater, A., Römling, U., Meyer, T. F., Tümmler, B., Gibbs, C. P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Blotting, Southern Chromosome Mapping Chromosomes, Bacterial DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Genes. Genome Molecular and cellular biology Molecular genetics Neisseria gonorrhoeae Neisseria gonorrhoeae - genetics Plasmids Restriction Mapping
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Beschreibung
Zusammenfassung:	Summary A macro‐restriction map of the Neisseria gonorrhoeae chromosome was constructed using the enzymes Nhel and Spel. Combinations of one‐and two‐dimensional electrophoresis of completely or partially digested chromosomal DNA were performed to align the restriction fragments. The chromosome is circular, with an estimated size of 2.33Mb±35kb. A genetic map was derived from the physical map; positions of over 60 defined loci were determined by Southern hybridization.
ISSN:	0950-382X 1365-2958
DOI:	10.1111/j.1365-2958.1991.tb02099.x