Difficult couplings in stepwise solid phase peptide synthesis: predictable or just a guess?
Stepwise solid phase peptide synthesis, Fmoc‐approach, of 88 peptides varying in length from 4 to 24 amino acid residues was performed using a uniform procedure for coupling, monitoring and deprotection steps. The data of 696 couplings have been incorporated into a computer programme in order to stu...
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Veröffentlicht in: | International Journal of Peptide and Protein Research 1991-08, Vol.38 (2), p.103-113 |
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Hauptverfasser: | , |
Format: | Artikel |
Sprache: | eng |
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Online-Zugang: | Volltext |
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Zusammenfassung: | Stepwise solid phase peptide synthesis, Fmoc‐approach, of 88 peptides varying in length from 4 to 24 amino acid residues was performed using a uniform procedure for coupling, monitoring and deprotection steps. The data of 696 couplings have been incorporated into a computer programme in order to study whether the degree of coupling can be predicted. Parameters studied are the nature of the amino acid to be coupled, the amino acid to be acylated and the length of the growing peptide chain. Using this information, prediction of “good” or “difficult” sequences, that is, peptides that can be synthesized without appreciable repeated couplings or the opposite, seems possible. |
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ISSN: | 0367-8377 1399-3011 |
DOI: | 10.1111/j.1399-3011.1991.tb01417.x |