Two Enzyme-Linked Immunosorbent Assay (ELISA) Systems for N1, N8-Diacetylspermidine and N1, N12-Diacetylspermine Using Monoclonal Antibodies
We obtained monoclonal antibodies against N1,N12-diacetylspermine (DiAcSpm) and N1, N8-diacetylspermidine (DiAcSpd), and developed two systems of competitive ELISA that utilize the antibodies and a common enzyme-labeled antigen to measure these diacetylpolyamines. Cross-reactions with N1-acetylsperm...
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Veröffentlicht in: | Journal of biochemistry (Tokyo) 2002-11, Vol.132 (5), p.783-788 |
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Sprache: | eng |
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Zusammenfassung: | We obtained monoclonal antibodies against N1,N12-diacetylspermine (DiAcSpm) and N1, N8-diacetylspermidine (DiAcSpd), and developed two systems of competitive ELISA that utilize the antibodies and a common enzyme-labeled antigen to measure these diacetylpolyamines. Cross-reactions with N1-acetylspermidine in the assay of DiAcSpm and with N8-acetylspermidine in the assay of DiAcSpd were as low as 0.26 and 0.6%, respectively, and were judged to be insignificant in clinical use for measuring urinary diacetylpolyamines. These assays were used to assess diurnal variations in diacetyl-polyamine excretion in urine to show that the excretion of diacetylpolyamines after normalization for the concentration of creatinine is stable over a day with only minimal diurnal variation. |
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ISSN: | 0021-924X |
DOI: | 10.1093/oxfordjournals.jbchem.a003287 |