Two Enzyme-Linked Immunosorbent Assay (ELISA) Systems for N1, N8-Diacetylspermidine and N1, N12-Diacetylspermine Using Monoclonal Antibodies

We obtained monoclonal antibodies against N1,N12-diacetylspermine (DiAcSpm) and N1, N8-diacetylspermidine (DiAcSpd), and developed two systems of competitive ELISA that utilize the antibodies and a common enzyme-labeled antigen to measure these diacetylpolyamines. Cross-reactions with N1-acetylsperm...

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Veröffentlicht in:Journal of biochemistry (Tokyo) 2002-11, Vol.132 (5), p.783-788
Hauptverfasser: Hamaoki, Masaru, Hiramatsu, Kyoko, Suzuki, Setsuo, Nagata, Atsuo, Kawakita, Masao
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Sprache:eng
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Zusammenfassung:We obtained monoclonal antibodies against N1,N12-diacetylspermine (DiAcSpm) and N1, N8-diacetylspermidine (DiAcSpd), and developed two systems of competitive ELISA that utilize the antibodies and a common enzyme-labeled antigen to measure these diacetylpolyamines. Cross-reactions with N1-acetylspermidine in the assay of DiAcSpm and with N8-acetylspermidine in the assay of DiAcSpd were as low as 0.26 and 0.6%, respectively, and were judged to be insignificant in clinical use for measuring urinary diacetylpolyamines. These assays were used to assess diurnal variations in diacetyl-polyamine excretion in urine to show that the excretion of diacetylpolyamines after normalization for the concentration of creatinine is stable over a day with only minimal diurnal variation.
ISSN:0021-924X
DOI:10.1093/oxfordjournals.jbchem.a003287