Localization of a DNA segment encompassing four tRNA genes to human chromosome 14q11 and its use as an anchor locus for linkage analysis

The chromosomal location of an 8.2-kb genomic fragment encompassing a cluster of four human tRNA genes has been determined by three complementary methods including Southern analysis of human/rodent somatic cell hybrids, in situ hybridization, and genetic linkage analysis. This tRNA cluster (TRP1, TR...

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Veröffentlicht in:	Genomics (San Diego, Calif.) Calif.), 1991-12, Vol.11 (4), p.1063-1070
Hauptverfasser:	Mitchell, Anna, Bale, Allen E., Wang-ge, Mary, Yi, Hua Fang, White, Ray, Pirtle, Robert M., McBride, O.Wesley
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Biological and medical sciences chromosome 14 Chromosome Mapping Chromosomes, Human, Pair 14 Classical genetics, quantitative genetics, hybrids Female Fundamental and applied biological sciences. Psychology genes Genetic Linkage Genetic Markers - genetics Genetics of eukaryotes. Biological and molecular evolution Human Humans Male Molecular Sequence Data Multigene Family Nucleic Acid Hybridization Polymorphism, Restriction Fragment Length restriction fragment length polymorphism Restriction Mapping RNA, Transfer - genetics RNA, Transfer, Leu - genetics RNA, Transfer, Pro - genetics RNA, Transfer, Thr - genetics
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Zusammenfassung:	The chromosomal location of an 8.2-kb genomic fragment encompassing a cluster of four human tRNA genes has been determined by three complementary methods including Southern analysis of human/rodent somatic cell hybrids, in situ hybridization, and genetic linkage analysis. This tRNA cluster (TRP1, TRP2, and TRL1) is located near the T-cell receptor α (TCRA) locus at 14q11, and several RFLPs were detected at this site. These RFLPs and those at the TCRA and MYH7 (cardiac β-MHC gene) loci have been used to type all informative members of the CEPH pedigrees. This has permitted ordering of these three gene loci and two anonymous probes (D14S26 and D14S25) in a 20-cM interval just below the centromere of chromosome 14. Based upon the chromosomal location and the polymorphisms at this site, one or more members of this gene cluster could serve as a useful anchor locus on chromosome 14.
ISSN:	0888-7543 1089-8646
DOI:	10.1016/0888-7543(91)90033-B