Incorporation of [1-14C]-linoleic acid by LLC-WRC256 tumour cells

The incorporation of [14C]‐linoleic acid (LA) into total lipid fractions was higher in LLC‐WRC256 cells from the log phase of growth as compared to those of the plateau phase. LA was mainly incorporated into the phospholipid (PL) fraction of cells during the log phase, whereas in the plateau phase it was mostly taken into cholesterol ester. The proportion of radioactivity was higher in phosphatidylserine of cells from the log phase, whereas in the plateau phase it was higher in phosphatidylcholine. This feature of LA incorporation may be an important factor in determining the proliferative capacity of tumour cells. Copyright © 2002 John Wiley & Sons, Ltd.