Spectrophotometric Determination of Yellow Pigment Content and Evaluation of Carotenoids by High-Performance Liquid Chromatography in Durum Wheat Grain

The so-called “yellow pigment” content of durum wheat has been used for a long time as an indicator of the color quality of durum wheat and pasta products. For decades the chemical nature of these pigments has been assigned to carotenoids, mainly to the xanthophyll lutein and its fatty acid esters. The chemical composition of the yellow pigments of eight German durum wheat cultivars was studied. Grains were milled on a laboratory mill. Pigment extraction of millstream fractions was performed according to the optimized ICC standard method 152 procedure, and the chemical composition of the extract was analyzed by isocratic reversed phase high-performance liquid chromatography. all-trans-Lutein ranged from 1.5 to 4 mg kg-1, and zeaxanthin was found in traces. No lutein esters and carotenes were detected. Surprisingly, the fraction of carotenoids of the complete yellow pigment content amounted to only 30−50% of the yellow pigment quantities, so there are still compounds in durum wheat not yet identified that contribute considerably to the yellow color of the grain extracts. The isolation and chemical identification of those pigments are under investigation. Keywords: Yellow pigment; ICC standard method 152; carotenoids; lutein; durum wheat