Quantitative Analysis of Osteonectin mRNA in Thyroid Carcinomas

Our recent study of the gene expression profile in thyroid carcinoma showed an overexpression of osteonectin mRNA, an extracellular matrix protein, in an anaplastic carcinoma. To confirm this, we measured the expression levels of osteonectin mRNA in 84 thyroid normal and tumor tissues, including fiv...

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Veröffentlicht in:	ENDOCRINE JOURNAL 2002, Vol.49(4), pp.511-516
Hauptverfasser:	TAKANO, TORU, HASEGAWA, YUKIKO, MIYAUCHI, AKIRA, MATSUZUKA, FUMIO, YOSHIDA, HIROSHI, KUMA, KANJI, HAYASHI, NOBUYASU, NAKAMORI, SHOJI, AMINO, NOBUYUKI
Format:	Artikel
Sprache:	eng
Schlagworte:	Carcinoma - chemistry Computer Systems Fine needle aspiration biopsy Humans Osteonectin Osteonectin - genetics Reverse Transcriptase Polymerase Chain Reaction Reverse transcription-PCR RNA, Messenger - analysis Thyroid carcinoma Thyroid Gland - chemistry Thyroid Neoplasms - chemistry Tumor Cells, Cultured
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Zusammenfassung:	Our recent study of the gene expression profile in thyroid carcinoma showed an overexpression of osteonectin mRNA, an extracellular matrix protein, in an anaplastic carcinoma. To confirm this, we measured the expression levels of osteonectin mRNA in 84 thyroid normal and tumor tissues, including five anaplastic carcinomas by realtime quantitative reverse-transcription PCR. Increased expression of osteonectin mRNA was observed in anaplastic carcinoma tissue. However, in five anaplastic carcinoma cell lines, no increase was observed in the expression levels of osteonectin mRNA. These findings suggest the possibility that increased expression of osteonectin mRNA in anaplastic carcinoma tissue may be due to its overexpression in stromal cells, but not in anaplastic carcinoma cells.
ISSN:	0918-8959 1348-4540
DOI:	10.1507/endocrj.49.511