Amitriptyline has a dual effect on the conductive properties of the epithelial Na channel

This study was undertaken with the aim of testing the action of amitriptyline on the epithelial Na channel (ENaC), which belongs to the same family (Deg/ENaC) as ASICs (acid‐sensing ion channels) and many other putative members in the brain. We assumed that, having a common protein structure, characterization of the amitriptyline‐ENaC interaction could help to elucidate the analgesic mechanism of this tricyclic antidepressant. Na‐channel characteristics were derived from the analysis of blocker‐induced lorentzian noise produced by amiloride. The effect of amitriptyline, present in the mucosal bathing solution, on the transepithelial short‐circuit current (1sc) and conductance (Gt), and on the blocker‐induced noise of apical Na channels, was studied on isolated ventral skin of the frog Rana ridibunda. Amitriptyline exerted a dual effect on the macroscopic short‐circuit current and conductance of the epithelia, increasing these two parameters in the concentration range 0.1–50 μM, while at higher concentrations (100–1000 μM) it showed an inhibitory action. The decrease in the association rate (k01) of amiloride to the apical Na channels from 15.6 ± 4.2 μM−1 S−1 in control Cl‐Ringer to 7.4 ± 1.7 μM−1 S−1 at 200 μM amitriptyline in a concentration‐dependent manner suggests a competitive binding of amitriptyline to the pyrazine ring binding site for amiloride.