High-cell-density cultivation of Escherichia coli
High-cell-density cultivations of Escherichia coli in glucose-mineral-salt media produce more than 100 g dry cells litre −1 in special fed-batch modes with feeding of glucose and ammonia only. The specific growth rate can be adjusted to allow optimum recombinant protein generation.
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| Veröffentlicht in: | Current opinion in biotechnology 1991-06, Vol.2 (3), p.380-384 |
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| Format: | Artikel |
| Sprache: | eng |
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| Online-Zugang: | Volltext |
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| Zusammenfassung: | High-cell-density cultivations of
Escherichia coli in glucose-mineral-salt media produce more than 100 g dry cells litre
−1 in special fed-batch modes with feeding of glucose and ammonia only. The specific growth rate can be adjusted to allow optimum recombinant protein generation. |
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| ISSN: | 0958-1669 1879-0429 |
| DOI: | 10.1016/S0958-1669(05)80142-9 |